Glycosylation is the most abundant and diverse form of post-translational modification of
proteins that is common to all eukaryotic cells. Enzymatic glycosylation of proteins involves a …

NGLY1/Ngly1 is a cytosolic peptide: N-glycanase, ie. de-N-glycosylating enzyme acting on
N-glycoproteins in mammals, generating free, unconjugated N-glycans and deglycosylated …

The cytoplasmic peptide: N-glycanase (Ngly1 in mammals) is a de-N-glycosylating enzyme
that is highly conserved among eukaryotes. It was recently reported that subjects harboring …

N-glycans on glycoproteins serve as one of the most important co-and post-translational
modifications of proteins, and it has been well established that they play pivotal roles in …

Cytosolic peptide: N-glycanase (PNGase/NGLY1 in mammals) catalyzes deglycosylation of
N-glycans on glycoproteins. A genetic disorder caused by mutations in the NGLY1 gene …

Oligosaccharyltransferases (OSTs) N-glycosylate proteins by transferring oligosaccharides
from lipid-linked oligosaccharides (LLOs) to asparaginyl residues of Asn-Xaa-Ser/Thr …

We recently reported increased levels of urinary free-glycans in some cancer patients. Here,
we focused on cancer related alterations in the levels of high molecular weight free-glycans …

Glycomics refers to the comprehensive analysis of glycans. Recent progress in
glycotechnology enables the determination of a variety of biological functions of glycans …

Cytosolic peptide: N-glycanase (NGLY1) is an enzyme that cleaves N-glycans from
glycoproteins that has been retrotranslocated from the endoplasmic reticulum (ER) lumen …

We developed a fluorescence‐quenching‐based assay system to determine the hydrolysis
activity of endo‐β‐N‐acetylglucosaminidases (ENGases). The pentasaccharide derivative 1 …