Live-cell imaging has opened an exciting window into the role cellular heterogeneity plays
in dynamic, living systems. A major critical challenge for this class of experiments is the …

It is tempting to believe that we now own the genome. The ability to read and rewrite it at will
has ushered in a stunning period in the history of science. Nonetheless, there is an Achilles' …

Plasmids are one of the most commonly used platforms for genetic engineering and
recombinant gene expression in bacteria. The range of available copy numbers for cloning …

The pace of modern science is staggering. The quantities of data now flowing from DNA
sequencers, fluorescence and electron microscopes, mass spectrometers, and other mind …

To perform their computational function, genetic circuits change states through a symphony
of genetic parts that turn regulator expression on and off. Debugging is frustrated by an …

Advances in DNA sequencing have revolutionized our ability to read genomes. However,
even in the most well-studied of organisms, the bacterium Escherichia coli, for≈ 65% of …

Evolution of gene regulation is crucial for our understanding of the phenotypic differences
between species, populations and individuals. Sequence-specific binding of transcription …

Allosteric regulation is found across all domains of life, yet we still lack simple, predictive
theories that directly link the experimentally tunable parameters of a system to its input …

Gene regulation relies on the specificity of transcription factor (TF)–DNA interactions. Limited
specificity may lead to crosstalk: a regulatory state in which a gene is either incorrectly …

Despite the central importance of transcriptional regulation in biology, it has proven difficult
to determine the regulatory mechanisms of individual genes, let alone entire gene networks …