[HTML][HTML] Macromolecular crowding in human tenocyte and skin fibroblast cultures: a comparative analysis
A Djalali-Cuevas, M Rettel, F Stein, M Savitski… - Materials today Bio, 2024 - Elsevier
Although human tenocytes and dermal fibroblasts have shown promise in tendon
engineering, no tissue engineered medicine has been developed due to the prolonged ex …
engineering, no tissue engineered medicine has been developed due to the prolonged ex …
[HTML][HTML] Macromolecular crowding in equine bone marrow mesenchymal stromal cell cultures using single and double hyaluronic acid macromolecules
Macromolecular crowding (MMC) enhances and accelerates extracellular matrix (ECM)
deposition in eukaryotic cell culture. Single hyaluronic acid (HA) molecules have not …
deposition in eukaryotic cell culture. Single hyaluronic acid (HA) molecules have not …
[HTML][HTML] Development of three-layer collagen scaffolds to spatially direct tissue-specific cell differentiation for enthesis repair
Enthesis repair remains a challenging clinical indication. Herein, a three-layer scaffold
composed of a tendon-like layer of collagen type I, a fibrocartilage-like layer of collagen type …
composed of a tendon-like layer of collagen type I, a fibrocartilage-like layer of collagen type …
The synergistic effect of physicochemical in vitro microenvironment modulators in human bone marrow stem cell cultures
Modern bioengineering utilises biomimetic cell culture approaches to control cell fate during
in vitro expansion. In this spirit, herein we assessed the influence of bidirectional surface …
in vitro expansion. In this spirit, herein we assessed the influence of bidirectional surface …
[PDF][PDF] Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
The combined effect of surface topography and substrate rigidity in stem cell cultures is still
under‐investigated, especially when biodegradable polymers are used. Herein, we …
under‐investigated, especially when biodegradable polymers are used. Herein, we …
Changes in physiological tendon substrate stiffness have moderate effects on tendon-derived cell growth and immune cell activation
Tendinopathy is characterised by pathological changes in tendon matrix composition,
architecture, and stiffness, alterations in tendon resident cell characteristics, and fibrosis …
architecture, and stiffness, alterations in tendon resident cell characteristics, and fibrosis …
Novel In Vitro Platform for Studying the Cell Response to Healthy and Diseased Tendon Matrices
Current in vitro models poorly represent the healthy or diseased tendon microenvironment,
limiting the translation of the findings to clinics. The present work aims to establish a …
limiting the translation of the findings to clinics. The present work aims to establish a …
[HTML][HTML] The interfascicular matrix of energy storing tendons houses heterogenous cell populations disproportionately affected by aging
DE Zamboulis, N Marr, L Lenzi, HL Birch… - Aging and …, 2024 - ncbi.nlm.nih.gov
Energy storing tendons such as the human Achilles and equine superficial digital flexor
tendon (SDFT) are prone to injury, with incidence increasing with aging, peaking in the 5 th …
tendon (SDFT) are prone to injury, with incidence increasing with aging, peaking in the 5 th …
Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture
The absence of a native extracellular matrix and the use of xenogeneic sera are often
associated with rapid tenocyte function losses during in vitro culture. Herein, we assessed …
associated with rapid tenocyte function losses during in vitro culture. Herein, we assessed …
[HTML][HTML] Xenogeneic versus allogeneic serum and macromolecular crowding in human tenocyte cultures
A Rampin, A Rossoni, L Chaniotaki, IS Gkiatas… - European Journal of …, 2024 - Elsevier
Allogeneic serum and tissue-specific extracellular matrix have been shown to maintain
permanently differentiated cell phenotype in culture. This is of particular importance for …
permanently differentiated cell phenotype in culture. This is of particular importance for …