Activity-based protein profiling (ABPP) is a technique that uses highly selective active-site
targeted chemical probes to label and monitor the state of proteins. ABPP integrates the …

The central dogma of modern biology underscores the pivotal roles proteins play in diverse
biological processes, the study of which necessitates advanced methods to produce …

Activity‐based E2 conjugating enzyme (E2)‐ubiquitin (Ub) probes have recently emerged as
effective tools for studying the molecular mechanism of E3 ligase (E3)‐catalyzed …

Herein we introduce vinylphosphonothiolates as a new class of cysteine-selective
electrophiles for protein labeling and the formation of stable protein–protein conjugates. We …

Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the
many enzymes involved in the ubiquitination system and the development of ubiquitination …

Conspectus Chemical protein synthesis has been proved as an efficient way to afford
medium-sized proteins with high homogeneity in workable quantities for various …

Advancement in our knowledge of deubiquitinases (DUBs) and their biological functions
requires biochemical tools permitting interrogation of DUB activities under physiologically …

Chemical protein synthesis and biorthogonal modification chemistries allow production of
unique proteins for a range of biological studies. Bond‐forming reactions for site‐selective …

Ubiquitin (UB) transfer cascades consisting of E1, E2, and E3 enzymes constitute a complex
network that regulates a myriad of biologic processes by modifying protein substrates …

Photocaged, activity‐based ubiquitin (Ub) probes (Ub‐ABPs) have been developed for the
time‐resolved probing of deubiquitinating enzyme (DUB) activities, but many Ub‐ABPs are …