Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Live-cell imaging powered by computation

H Shroff, I Testa, F Jug, S Manley - Nature Reviews Molecular Cell …, 2024 - nature.com
The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

An effective feature engineering for DNN using hybrid PCA-GWO for intrusion detection in IoMT architecture

SP RM, PKR Maddikunta, M Parimala, S Koppu… - Computer …, 2020 - Elsevier
The entire computing paradigm is changed due to the technological advancements in
Information and Communication Technology (ICT). Due to these advancements, various …

Deep learning enables fast and dense single-molecule localization with high accuracy

A Speiser, LR Müller, P Hoess, U Matti, CJ Obara… - Nature …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …

Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging

S Fu, W Shi, T Luo, Y He, L Zhou, J Yang, Z Yang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …

Self-supervised machine learning pushes the sensitivity limit in label-free detection of single proteins below 10 kDa

M Dahmardeh, H Mirzaalian Dastjerdi, H Mazal… - Nature …, 2023 - nature.com
Interferometric scattering (iSCAT) microscopy is a label-free optical method capable of
detecting single proteins, localizing their binding positions with nanometer precision, and …

Deep learning in single-molecule microscopy: fundamentals, caveats, and recent developments

L Möckl, AR Roy, WE Moerner - Biomedical optics express, 2020 - opg.optica.org
Deep learning-based data analysis methods have gained considerable attention in all fields
of science over the last decade. In recent years, this trend has reached the single-molecule …

Single molecule fluorescence imaging of nanoconfinement in porous materials

B Dong, N Mansour, TX Huang, W Huang… - Chemical Society …, 2021 - pubs.rsc.org
This review covers recent progress in using single molecule fluorescence microscopy
imaging to understand the nanoconfinement in porous materials. The single molecule …

Quantitative single-molecule localization microscopy

S Hugelier, PL Colosi… - Annual Review of …, 2023 - annualreviews.org
Super-resolution fluorescence microscopy allows the investigation of cellular structures at
nanoscale resolution using light. Current developments in super-resolution microscopy have …

Single-molecule fluorescence imaging for probing nanocatalytic process

Y Xiao, W Xu - Chem, 2023 - cell.com
Single-molecule fluorescent microscopy (SMFM) has proved to be a powerful imaging tool
for in situ monitoring catalytic reactions on individual nanocatalysts with high spatiotemporal …