Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …

CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years,
however, the translation of this biotechnology into therapy has been hindered by efficient …

Prime editors have a broad range of potential research and clinical applications. However,
methods to delineate their genome-wide editing activities have generally relied on indirect …

Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic
perturbations because they can process multiple guide RNAs expressed as a single …

Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits
relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered …

Multiplexed modulation of endogenous genes is crucial for sophisticated gene therapy and
cell engineering. CRISPR–Cas12a systems enable versatile multiple-genomic-loci targeting …

Zebrafish (Danio rerio) is a valuable non-mammalian vertebrate model widely used to study
development and disease, including more recently cancer. The evolutionary conservation of …

Current CRISPR-Cas-based nucleic acid sensing methods relying on the preassembled
Cas-crRNA complexes are generally limited to the detection of protospacer-adjacent motif …

Abstract Analysis of off-target editing is an important aspect of the development of safe
nuclease-based genome editing therapeutics. in vivo assessment of nuclease off-target …

Current programmable nuclease-based methods (for example, CRISPR–Cas9) for the
precise correction of a disease-causing genetic mutation harness the homology-directed …