Molecular mechanisms of CRISPR-Cas immunity in bacteria

PM Nussenzweig, LA Marraffini - Annual review of genetics, 2020 - annualreviews.org
Prokaryotes have developed numerous defense strategies to combat the constant threat
posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced …

Evolutionary plasticity and functional versatility of CRISPR systems

EV Koonin, KS Makarova - PLoS Biology, 2022 - journals.plos.org
The principal biological function of bacterial and archaeal CRISPR systems is RNA-guided
adaptive immunity against viruses and other mobile genetic elements (MGEs). These …

Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering

H Altae-Tran, S Kannan, AJ Suberski, KS Mears… - Science, 2023 - science.org
Microbial systems underpin many biotechnologies, including CRISPR, but the exponential
growth of sequence databases makes it difficult to find previously unidentified systems. In …

Alternative functions of CRISPR–Cas systems in the evolutionary arms race

P Mohanraju, C Saha, P van Baarlen… - Nature Reviews …, 2022 - nature.com
CRISPR–Cas systems of bacteria and archaea comprise chromosomal loci with typical
repetitive clusters and associated genes encoding a range of Cas proteins. Adaptation of …

CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids

R Pinilla-Redondo, J Russel… - Nucleic acids …, 2022 - academic.oup.com
Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile
genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components …

The CRISPR-Cas mechanism for adaptive immunity and alternate bacterial functions fuels diverse biotechnologies

S Newsom, HP Parameshwaran, L Martin… - Frontiers in cellular and …, 2021 - frontiersin.org
Bacterial and archaeal CRISPR-Cas systems offer adaptive immune protection against
foreign mobile genetic elements (MGEs). This function is regulated by sequence specific …

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation

TY Liu, JA Doudna - Journal of Biological Chemistry, 2020 - ASBMB
Among the multiple antiviral defense mechanisms found in prokaryotes, CRISPR-Cas
systems stand out as the only known RNA-programmed pathways for detecting and …

Endogenous type I CRISPR-Cas: from foreign DNA defense to prokaryotic engineering

Y Zheng, J Li, B Wang, J Han, Y Hao… - … in bioengineering and …, 2020 - frontiersin.org
Establishment of production platforms through prokaryotic engineering in microbial
organisms would be one of the most efficient means for chemicals, protein, and biofuels …

Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment

N Cui, JT Zhang, Y Liu, Y Liu, XY Liu, C Wang… - Molecular Cell, 2023 - cell.com
Summary Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit
a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types …

Genome-wide CRISPR screen identifies genes synthetically lethal with GRA17, a nutrient channel encoding gene in Toxoplasma

TC Paredes-Santos, MA Bitew, C Swale… - PLoS …, 2023 - journals.plos.org
Toxoplasma gondii is a parasite that replicates within a specialized compartment called the
parasitophorous vacuole (PV), which is surrounded by the PV membrane (PVM). To obtain …