Electrically switchable nanolever technology for the screening of metal-chelating peptides in hydrolysates

S El Hajj, CT Sepulveda Rincon… - Journal of Agricultural …, 2021 - ACS Publications
Metal-chelating peptides (MCP) are considered as indirect antioxidants due to their capacity
to inhibit radical chain reaction and oxidation. Here, we propose a new proof of concept for …

Extending the competitive threshold collision-induced dissociation of Zn (II) ternary complexes using traveling-wave ion mobility-mass spectrometry

KN Senyah, P Asare, JD Wilcox, F Angiolari… - International Journal of …, 2023 - Elsevier
The competitive threshold collision-induced dissociation (TCID) of the ternary complex [amb
5+ Zn (II)+ NTA]−→[amb 5+ Zn (II)]−+ NTA or→[NTA+ Zn (II)]−+ amb 5, where amb 5= His …

Gas-phase Ni (II) affinities of alternative metal binding peptides from competitive threshold collision-induced dissociation

P Asare, KN Senyah, JD Wilcox, J Morales… - International Journal of …, 2024 - Elsevier
The Ni (II) affinity of the polyhistidine tag is used in the purification of recombinant proteins
by immobilized metal affinity chromatography. Here we measured the relative gas-phase Ni …

Formation of Co (II), Ni (II), Zn (II) complexes of alternative metal binding heptapeptides and nitrilotriacetic acid: Discovering new potential affinity tags

AA Flores, OS Falokun, AB Ilesanmi… - International Journal of …, 2021 - Elsevier
Using electrospray ionization-ion mobility-mass spectrometry (ESI-IM-MS) a new series of
alternative metal binding-5 (amb 5) heptapeptides that provide various combinations of His …

pH dependent chelation study of Zn (II) and Ni (II) by a series of hexapeptides using electrospray ionization–Ion mobility–Mass spectrometry

AB Ilesanmi, TC Moore, LA Angel - International Journal of Mass …, 2020 - Elsevier
Presented here are the results of a study characterizing the selective metal chelating
performance of the alternative metal binding (amb) peptide: acetyl-His 1-Cys 2-Gly 3-Pro 4 …

ESI-IM-MS reveals the metal binding of three analog methanobactin peptides with different numbers of free Cys at physiological pH

R Vangala, LA Angel - International Journal of Mass Spectrometry, 2021 - Elsevier
Three analog methanobactin (amb 7) peptides containing the primary structure acetyl-Leu 1-
His 2-Cys 3-Gly 4-Ser 5-Cys 6-Tyr 7-Pro 8-His 9-Cys 10-Ser 11-Cys 12-Met 13 but differing …

Thermochemical studies of Ni (II) and Zn (II) ternary complexes using ion mobility-mass spectrometry

AJ Corrales, AV Arredondo, AA Flores… - JoVE (Journal of …, 2022 - jove.com
This article describes an experimental protocol using electrospray-ion mobility-mass
spectrometry (ES-IM-MS) and energy-resolved threshold collision-induced dissociation …

Ion Mobility Mass Spectrometry Analysis of Alternative Metal Binding Peptides for the Utilization of Cloning, Overexpression and Purification of Affinity Tagged …

A Flores - 2022 - search.proquest.com
Affinity tags have many applications; they can be used for tracking proteins, improving
solubility, and are a tool in protein purification of recombinant proteins. Recombinant …