CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
[HTML][HTML] Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …
9) shows the opportunity to treat a diverse array of untreated various genetic and …
Human genetic diversity alters off-target outcomes of therapeutic gene editing
CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat
disease. However, standard computational and biochemical methods to predict off-target …
disease. However, standard computational and biochemical methods to predict off-target …
Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes
NP Kieu, M Lenman, ES Wang, BL Petersen… - Scientific Reports, 2021 - nature.com
The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide
use in agriculture. However, in potato breeding, increased resistance obtained via …
use in agriculture. However, in potato breeding, increased resistance obtained via …
DeepCRISPR: optimized CRISPR guide RNA design by deep learning
A major challenge for effective application of CRISPR systems is to accurately predict the
single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would …
single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would …
CRISPR/Cpf1-mediated DNA-free plant genome editing
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and
induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA …
induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA …
Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
[HTML][HTML] Reduced enzymatic browning in potato tubers by specific editing of a polyphenol oxidase gene via ribonucleoprotein complexes delivery of the CRISPR/Cas9 …
MN González, GA Massa, M Andersson… - Frontiers in Plant …, 2020 - frontiersin.org
Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones,
leading to the formation of dark-colored precipitates in fruits and vegetables. This process …
leading to the formation of dark-colored precipitates in fruits and vegetables. This process …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …