Highlights•Harnessing cellular processes to produce valuable metabolic products is of
growing economic importance.•However, biological complexity necessitates many attempts …

Biotechnological production of phenolic acids is attracting increased interest due to their
superior antioxidant activity, as well as other antimicrobial, dietary, and health benefits. As …

Engineering cells to produce valuable metabolic products is hindered by the slow and
laborious methods available for evaluating product concentration. Consequently, many …

Pseudomonas putida KT2440 has received increasing attention as an important biocatalyst
for the conversion of diverse carbon sources to multiple products, including the olefinic …

Abstract Adaptive Laboratory Evolution (ALE) is a powerful tool for engineering and
understanding microbial physiology. ALE relies on the selection and enrichment of …

Abstract Within the Design-Build-Test Cycle for strain engineering, rapid product detection
and selection strategies remain challenging and limit overall throughput. Here we …

Highlights•Transcription factors are approved sensors to monitor intracellular
concentrations.•Sensors coupled to gfp expression convert concentrations into a graded …

Monitoring cellular behavior and eventually properly adapting cellular processes is key to
handle the enormous complexity of today's metabolic engineering questions. Hence …

Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic
engineering, and production of industrial enzymes. To fully take advantage of this organism …

Promoter engineering is an enabling technology in metabolic engineering and synthetic
biology. As an indispensable part of synthetic biology, the promoter is a key factor in …