A fundamental challenge in biological research is achieving an atomic-level description and
mechanistic understanding of the function of biomolecules. Techniques for biomolecular …

Integrative structure determination is a powerful approach to modeling the structures of
biological systems based on data produced by multiple experimental and theoretical …

Highlights•The principles of protein structural ensemble determination are described.•The
use of experimental data averaged over multiple states is analysed.•Approaches for …

Three-dimensional (3D) structure determination by single-particle analysis of cryo-electron
microscopy (cryo-EM) images requires many parameters to be determined from extremely …

The paradigmatic disordered protein tau plays an important role in neuronal function and
neurodegenerative diseases. To disentangle the factors controlling the balance between …

Deep learning models, such as AlphaFold2 and RosettaFold, enable high-accuracy protein
structure prediction. However, large protein complexes are still challenging to predict due to …

This Meeting Review describes the proceedings and conclusions from the inaugural
meeting of the Electron Microscopy Validation Task Force organized by the Unified Data …

The nuclear Overhauser effect (NOE) is defined as the change in intensity of one NMR
resonance that occurs when another is saturated. It results from dipole–dipole cross …

Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural
insights into protein conformation and protein-protein interactions. Its medium-resolution …

The voltage-dependent anion channel (VDAC), also known as mitochondrial porin, is the
most abundant protein in the mitochondrial outer membrane (MOM). VDAC is the channel …