Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

RNA granules are mesoscale assemblies that form in the absence of limiting membranes.
RNA granules contain factors for RNA biogenesis and turnover and are often assumed to …

The nucleolus is the largest biomolecular condensate and facilitates transcription,
processing, and assembly of ribosomal RNA (rRNA). Although nucleolar function is thought …

Advances in cryo-electron tomography (cryo-ET) have produced new opportunities to
visualize the structures of dynamic macromolecules in native cellular environments. While …

Cellular cryo-electron tomography (cryo-ET) enables three-dimensional reconstructions of
organelles in their native cellular environment at subnanometer resolution. However …

Chaperonins are large barrel-shaped complexes that mediate ATP-dependent protein
folding,–. The bacterial chaperonin GroEL forms juxtaposed rings that bind unfolded protein …

Cryo-electron tomography (cryo-ET) is widely used to explore the 3D density of
biomacromolecules. However, the heavy noise and missing wedge effect prevent directly …

In situ cryo-electron tomography enables investigation of macromolecules in their native
cellular environment. Samples have become more readily available owing to recent …

Biology spans a continuum of length and time scales. Individual experimental methods only
glimpse discrete pieces of this spectrum but can be combined to construct a more holistic …

Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex
biological specimens such as viral particles, cellular sections and, in some cases, whole …