The demand for high-throughput data collection in electron microscopy is increasing for
applications in structural and cellular biology. Here we present a combination of software …

Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Abstract The β1-adrenoceptor (β1AR) is a G-protein-coupled receptor (GPCR) that couples
to the heterotrimeric G protein Gs. G-protein-mediated signalling is terminated by …

The recent rapid development of single-particle electron cryo-microscopy (cryo-EM) now
allows structures to be solved by this method at resolutions close to 3 Å. Here, a number of …

As part of its remit to provide computational support to the cryo-EM community, the
Collaborative Computational Project for Electron cryo-Microscopy (CCP-EM) has produced …

The calcium-activated chloride channel TMEM16A is a ligand-gated anion channel that
opens in response to an increase in intracellular Ca2+ concentration,,. The protein is …

Electron tomography of frozen, hydrated samples allows structure determination of
macromolecular complexes that are embedded in complex environments. Provided that the …

The pyruvate dehydrogenase complex (PDHc) links glycolysis to the citric acid cycle by
converting pyruvate into acetyl-coenzyme A. PDHc encompasses three enzymatically active …

Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well
as impacting microbial ecology, physiology and evolution, phages are exploited as tools in …

Abstract Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding
protein, binds to exposed ssDNA to protect it from nucleases, participates in a myriad of …