Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling

P Bourceau, B Geier, V Suerdieck, T Bien… - Nature …, 2023 - nature.com
Label-free molecular imaging techniques such as matrix-assisted laser desorption ionization
mass spectrometry imaging (MALDI-MSI) enable the direct and simultaneous mapping of …

Phylogenetic identification and in situ detection of individual microbial cells without cultivation

RI Amann, W Ludwig, KH Schleifer - Microbiological reviews, 1995 - Am Soc Microbiol
The frequent discrepancy between direct microscopic counts and numbers of culturable
bacteria from environmental samples is just one of several indications that we currently …

Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing

S Takahashi, J Tomita, K Nishioka, T Hisada… - PloS one, 2014 - journals.plos.org
For the analysis of microbial community structure based on 16S rDNA sequence diversity,
sensitive and robust PCR amplification of 16S rDNA is a critical step. To obtain accurate …

[Retracted] Microbial Ecology of Anaerobic Digesters: The Key Players of Anaerobiosis

F Ali Shah, Q Mahmood, M Maroof Shah… - The Scientific World …, 2014 - Wiley Online Library
Anaerobic digestion is the method of wastes treatment aimed at a reduction of their
hazardous effects on the biosphere. The mutualistic behavior of various anaerobic …

The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set

H Daims, A Brühl, R Amann, KH Schleifer… - Systematic and applied …, 1999 - Elsevier
In situ hybridization with rRNA-targeted oligonucleotide probes has become a widely
applied tool for direct analysis of microbial population structures of complex natural and …

Group‐specific primer and probe sets to detect methanogenic communities using quantitative real‐time polymerase chain reaction

Y Yu, C Lee, J Kim, S Hwang - Biotechnology and …, 2005 - Wiley Online Library
Real‐time polymerase chain reaction (PCR) is a highly sensitive method that can be used
for the detection and quantification of microbial populations without cultivating them in …

Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO)

R Conrad - Microbiological reviews, 1996 - Am Soc Microbiol
Production and consumption processes in soils contribute to the global cycles of many trace
gases (CH4, CO, OCS, H2, N2O, and NO) that are relevant for atmospheric chemistry and …

Bias in template-to-product ratios in multitemplate PCR

MF Polz, CM Cavanaugh - Applied and environmental …, 1998 - Am Soc Microbiol
Bias introduced by the simultaneous amplification of specific genes from complex mixtures
of templates remains poorly understood. To explore potential causes and the extent of bias …

Exploring deep microbial life in coal-bearing sediment down to~ 2.5 km below the ocean floor

F Inagaki, KU Hinrichs, Y Kubo, MW Bowles, VB Heuer… - Science, 2015 - science.org
Microbial life inhabits deeply buried marine sediments, but the extent of this vast ecosystem
remains poorly constrained. Here we provide evidence for the existence of microbial …

Taxonomic, phylogenetic, and ecological diversity of methanogenic Archaea

JL Garcia, BKC Patel, B Ollivier - Anaerobe, 2000 - Elsevier
1Laboratoire de Microbiologie IRD, Universite de Provence, ESIL case 925, 163 Avenue
de Luminy, 13288 Marseille cedex 9, France 2Microbial Discovery Research Unit, School of …