mRNAs, proteins and the emerging principles of gene expression control
C Buccitelli, M Selbach - Nature Reviews Genetics, 2020 - nature.com
Gene expression involves transcription, translation and the turnover of mRNAs and proteins.
The degree to which protein abundances scale with mRNA levels and the implications in …
The degree to which protein abundances scale with mRNA levels and the implications in …
Time-resolved single-cell RNA-seq using metabolic RNA labelling
Single-cell RNA genomics technologies are revolutionizing biomedical science by profiling
single cells with unprecedented resolution, providing fundamental insights into the role of …
single cells with unprecedented resolution, providing fundamental insights into the role of …
Thiol-linked alkylation of RNA to assess expression dynamics
Gene expression profiling by high-throughput sequencing reveals qualitative and
quantitative changes in RNA species at steady state but obscures the intracellular dynamics …
quantitative changes in RNA species at steady state but obscures the intracellular dynamics …
Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
Y Enuka, M Lauriola, ME Feldman… - Nucleic acids …, 2016 - academic.oup.com
Circular RNAs (circRNAs) are widespread circles of non-coding RNAs with largely unknown
function. Because stimulation of mammary cells with the epidermal growth factor (EGF) …
function. Because stimulation of mammary cells with the epidermal growth factor (EGF) …
Exon circularization requires canonical splice signals
S Starke, I Jost, O Rossbach, T Schneider, S Schreiner… - Cell reports, 2015 - cell.com
Circular RNAs (circRNAs), an abundant class of noncoding RNAs in higher eukaryotes, are
generated from pre-mRNAs by circularization of adjacent exons. Using a set of 15 circRNAs …
generated from pre-mRNAs by circularization of adjacent exons. Using a set of 15 circRNAs …
Nascent RNA analyses: tracking transcription and its regulation
The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …
genome. Decoding of this information begins with regulated transcription of genomic DNA …
[HTML][HTML] Splicing kinetics and coordination revealed by direct nascent RNA sequencing through nanopores
HL Drexler, K Choquet, LS Churchman - Molecular cell, 2020 - cell.com
Understanding how splicing events are coordinated across numerous introns in metazoan
RNA transcripts requires quantitative analyses of transient RNA processing events in living …
RNA transcripts requires quantitative analyses of transient RNA processing events in living …
The spliceosome is a therapeutic vulnerability in MYC-driven cancer
MYC (also known as c-MYC) overexpression or hyperactivation is one of the most common
drivers of human cancer. Despite intensive study, the MYC oncogene remains recalcitrant to …
drivers of human cancer. Despite intensive study, the MYC oncogene remains recalcitrant to …
Global quantification of mammalian gene expression control
B Schwanhäusser, D Busse, N Li, G Dittmar… - Nature, 2011 - nature.com
Gene expression is a multistep process that involves the transcription, translation and
turnover of messenger RNAs and proteins. Although it is one of the most fundamental …
turnover of messenger RNAs and proteins. Although it is one of the most fundamental …
Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens
We present a combinatorial indexing method, PerturbSci-Kinetics, for capturing whole
transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across …
transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across …