The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …

The bacterium Deinococcus radiodurans shows remarkable resistance to a range of
damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, and …

Bronchioalveolar stem cells (BASC s) are a potential source for lung regeneration, but direct
in vivo evidence for a multipotential lineage contribution during homeostasis and disease is …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

Several members of the Rubiaceae and Violaceae families produce a series of cyclotides or
macrocyclic peptides of 29–31 amino acids with an embedded cystine knot. We aim to …

Biomoleküle in ihrer natürlichen Umgebung zu erforschen, ist wegen der enormen
Komplexität zellulärer Systeme eine anspruchsvolle Aufgabe. In den letzten Jahren …

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme
(Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE …

Biophysical techniques such as fluorescence spectroscopy and nuclear magnetic
resonance (NMR) spectroscopy provide a window into the inner workings of proteins. These …

A new enzymatic protein ligation tool, sortase, has recently emerged from Gram‐positive
bacteria. This article outlines the technique, sortase‐mediated ligation, and its applications …

Circular proteins are a recently discovered phenomenon. They presumably evolved to
confer advantages over ancestral linear proteins while maintaining the intrinsic biological …