Polarisation optics for biomedical and clinical applications: a review

C He, H He, J Chang, B Chen, H Ma… - Light: Science & …, 2021 - nature.com
Many polarisation techniques have been harnessed for decades in biological and clinical
research, each based upon measurement of the vectorial properties of light or the vectorial …

A guide to single-particle tracking

F Simon, LE Weiss, S van Teeffelen - Nature Reviews Methods Primers, 2024 - nature.com
Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …

[HTML][HTML] Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution

LH Yeh, IE Ivanov, T Chandler, JR Byrum, BB Chhun… - Nature …, 2024 - nature.com
The dry mass and the orientation of biomolecules can be imaged without a label by
measuring their permittivity tensor (PT), which describes how biomolecules affect the phase …

Contractility, focal adhesion orientation, and stress fiber orientation drive cancer cell polarity and migration along wavy ECM substrates

RS Fischer, X Sun, MA Baird… - Proceedings of the …, 2021 - National Acad Sciences
Contact guidance is a powerful topographical cue that induces persistent directional cell
migration. Healthy tissue stroma is characterized by a meshwork of wavy extracellular matrix …

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

K Zhanghao, W Liu, M Li, Z Wu, X Wang, X Chen… - Nature …, 2020 - nature.com
Lipid membranes are found in most intracellular organelles, and their heterogeneities play
an essential role in regulating the organelles' biochemical functionalities. Here we report a …

[HTML][HTML] Computerized cell tracking: Current methods, tools and challenges

N Emami, Z Sedaei, R Ferdousi - Visual Informatics, 2021 - Elsevier
In developmental biology, knowledge of cell structure and their (morpho) dynamic behavior,
leads to a comprehensive understanding of their conducts and the mechanisms in which …

Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy

K Zhanghao, X Chen, W Liu, M Li, Y Liu, Y Wang… - Nature …, 2019 - nature.com
Fluorescence polarization microscopy images both the intensity and orientation of
fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of …

Membrane reshaping by micrometric curvature sensitive septin filaments

A Beber, C Taveneau, M Nania, FC Tsai… - Nature …, 2019 - nature.com
Septins are cytoskeletal filaments that assemble at the inner face of the plasma membrane.
They are localized at constriction sites and impact membrane remodeling. We report in vitro …

Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions

V Swaminathan, JM Kalappurakkal… - Proceedings of the …, 2017 - National Acad Sciences
Integrins are transmembrane receptors that, upon activation, bind extracellular ligands and
link them to the actin filament (F-actin) cytoskeleton to mediate cell adhesion and migration …

Polarization microscopy: from ensemble structural imaging to single-molecule 3D orientation and localization microscopy

S Brasselet, MA Alonso - Optica, 2023 - opg.optica.org
Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …