Coordination of the cell cycle in trypanosomes

RJ Wheeler, K Gull, JD Sunter - Annual review of microbiology, 2019 - annualreviews.org
Trypanosomes have complex life cycles within which there are both proliferative and
differentiation cell divisions. The coordination of the cell cycle to achieve these different …

Life in plastic, it's fantastic! How Leishmania exploit genome instability to shape gene expression

JA Black, JL Reis-Cunha, AK Cruz… - Frontiers in cellular and …, 2023 - frontiersin.org
Leishmania are kinetoplastid pathogens that cause leishmaniasis, a debilitating and
potentially life-threatening infection if untreated. Unusually, Leishmania regulate their gene …

FANCM, BRCA1, and BLM cooperatively resolve the replication stress at the ALT telomeres

X Pan, WC Drosopoulos, L Sethi… - Proceedings of the …, 2017 - National Acad Sciences
In the mammalian genome, certain genomic loci/regions pose greater challenges to the
DNA replication machinery (ie, the replisome) than others. Such known genomic loci/regions …

Transcriptionally driven DNA replication program of the human parasite Leishmania major

R Lombraña, A Álvarez, JM Fernández-Justel… - Cell reports, 2016 - cell.com
Faithful inheritance of eukaryotic genomes requires the orchestrated activation of multiple
DNA replication origins (ORIs). Although origin firing is mechanistically conserved, how …

Shining a spotlight on DNA: single-molecule methods to visualise DNA

G Kaur, JS Lewis, AM van Oijen - Molecules, 2019 - mdpi.com
The ability to watch single molecules of DNA has revolutionised how we study biological
transactions concerning nucleic acids. Many strategies have been developed to manipulate …

Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe

CA Marques, NJ Dickens, D Paape, SJ Campbell… - Genome biology, 2015 - Springer
Background DNA replication initiates on defined genome sites, termed origins. Origin usage
appears to follow common rules in the eukaryotic organisms examined to date: all …

Integrative analysis of the Trypanosoma brucei gene expression cascade predicts differential regulation of mRNA processing and unusual control of ribosomal …

EB Antwi, JR Haanstra, G Ramasamy, B Jensen… - BMC genomics, 2016 - Springer
Background Trypanosoma brucei is a unicellular parasite which multiplies in mammals
(bloodstream form) and Tsetse flies (procyclic form). Trypanosome RNA polymerase II …

Differences in the detection of BrdU/EdU incorporation assays alter the calculation for G1, S, and G2 phases of the cell cycle in trypanosomatids

MS da Silva, PAM Muñoz, HA Armelin… - Journal of Eukaryotic …, 2017 - Wiley Online Library
Trypanosomatids are the etiologic agents of various infectious diseases in humans. They
diverged early during eukaryotic evolution and have attracted attention as peculiar models …

[HTML][HTML] Nuclear DNA replication in trypanosomatids: there are no easy methods for solving difficult problems

MS da Silva, RS Pavani, JD Damasceno… - Trends in …, 2017 - cell.com
In trypanosomatids, etiological agents of devastating diseases, replication is robust and
finely controlled to maintain genome stability and function in stressful environments …

Read, write, adapt: challenges and opportunities during kinetoplastid genome replication

JD Damasceno, CA Marques, J Black, E Briggs… - Trends in Genetics, 2021 - cell.com
The genomes of all organisms are read throughout their growth and development,
generating new copies during cell division and encoding the cellular activities dictated by …