Anionic phospholipids control mechanisms of GPCR-G protein recognition
G protein-coupled receptors (GPCRs) are embedded in phospholipids that strongly
influence drug-stimulated signaling. Anionic lipids are particularly important for GPCR …
influence drug-stimulated signaling. Anionic lipids are particularly important for GPCR …
Advances in nanodisc platforms for membrane protein purification
JM Pettersen, Y Yang, AS Robinson - Trends in Biotechnology, 2023 - cell.com
Membrane scaffold protein nanodiscs (MSPNDs) are an invaluable tool for improving
purified membrane protein (MP) stability and activity compared to traditional micellar …
purified membrane protein (MP) stability and activity compared to traditional micellar …
Dual mechanisms of cholesterol-GPCR interactions that depend on membrane phospholipid composition
Cholesterol is a critical component of mammalian cell membranes and an allosteric
modulator of G protein-coupled receptors (GPCRs), but divergent views exist on the …
modulator of G protein-coupled receptors (GPCRs), but divergent views exist on the …
Single-molecule visualization of human A2A adenosine receptor activation by a G protein and constitutively activating mutations
Mutations that constitutively activate G protein-coupled receptors (GPCRs), known as
constitutively activating mutations (CAMs), modify cell signaling and interfere with drugs …
constitutively activating mutations (CAMs), modify cell signaling and interfere with drugs …
Single-molecule analysis reveals that a glucagon-bound extracellular domain of the glucagon receptor is dynamic
T Liu, S Khanal, GD Hertslet, R Lamichhane - Journal of Biological …, 2023 - ASBMB
Dynamic information is vital to understanding the activation mechanism of G protein-coupled
receptors (GPCRs). Despite the availability of high-resolution structures of different …
receptors (GPCRs). Despite the availability of high-resolution structures of different …
Global insights into the fine tuning of human A2AAR conformational dynamics in a ternary complex with an engineered G protein viewed by NMR
G protein-coupled receptor (GPCR) conformational plasticity enables formation of ternary
signaling complexes with intracellular proteins in response to binding extracellular ligands …
signaling complexes with intracellular proteins in response to binding extracellular ligands …
[HTML][HTML] Membrane mimetic-dependence of GPCR energy landscapes
We leveraged variable-temperature 19 F-NMR spectroscopy to compare the conformational
equilibria of the human A 2A adenosine receptor (A 2A AR), a class AG protein-coupled …
equilibria of the human A 2A adenosine receptor (A 2A AR), a class AG protein-coupled …
Functional GPCR expression in eukaryotic LEXSY system
A Luginina, I Maslov, P Khorn, O Volkov… - Journal of Molecular …, 2023 - Elsevier
G protein-coupled receptors (GPCRs) form the largest superfamily of membrane proteins in
the human genome, and represent one of the most important classes of drug targets. Their …
the human genome, and represent one of the most important classes of drug targets. Their …
Exploring GPCR conformational dynamics using single-molecule fluorescence
E Agyemang, AN Gonneville, S Tiruvadi-Krishnan… - Methods, 2024 - Elsevier
G protein-coupled receptors (GPCRs) are membrane proteins that transmit specific external
stimuli into cells by changing their conformation. This conformational change allows them to …
stimuli into cells by changing their conformation. This conformational change allows them to …
Biophysical Dissection of Isolated GPCRs: The Adenosine A2A Receptor under the Bistouries
JL Banères, T Botzanowski, JA Boutin, B Calamini… - Receptors, 2023 - mdpi.com
In an effort to provide an overview of the biophysical approaches used to study G-protein-
coupled receptors, we chose to consider the adenosine A2A receptor as a model, as it is …
coupled receptors, we chose to consider the adenosine A2A receptor as a model, as it is …