Mechanisms and pathology of protein misfolding and aggregation

N Louros, J Schymkowitz, F Rousseau - Nature Reviews Molecular Cell …, 2023 - nature.com
Despite advances in machine learning-based protein structure prediction, we are still far
from fully understanding how proteins fold into their native conformation. The conventional …

Targeting biomolecular condensation and protein aggregation against cancer

JL Silva, D Foguel, VF Ferreira, TCRG Vieira… - Chemical …, 2023 - ACS Publications
Biomolecular condensates, membrane-less entities arising from liquid–liquid phase
separation, hold dichotomous roles in health and disease. Alongside their physiological …

Liquid-liquid phase separation of α-synuclein: a new mechanistic insight for α-synuclein aggregation associated with Parkinson's disease pathogenesis

S Mukherjee, A Sakunthala, L Gadhe… - Journal of molecular …, 2023 - Elsevier
Aberrant aggregation of the misfolded presynaptic protein, α-Synuclein (α-Syn) into Lewy
body (LB) and Lewy neuritis (LN) is a major pathological hallmark of Parkinson's disease …

Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation

A Joshi, A Walimbe, A Avni, SK Rai, L Arora… - Nature …, 2023 - nature.com
Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …

Wetting and complex remodeling of membranes by biomolecular condensates

A Mangiarotti, N Chen, Z Zhao, R Lipowsky… - Nature …, 2023 - nature.com
Cells compartmentalize parts of their interiors into liquid-like condensates, which can be
reconstituted in vitro. Although these condensates interact with membrane-bound …

Mass photometric detection and quantification of nanoscale α-synuclein phase separation

S Ray, TO Mason, L Boyens-Thiele, A Farzadfard… - Nature …, 2023 - nature.com
Protein liquid–liquid phase separation can lead to disease-related amyloid fibril formation.
The mechanisms of conversion of monomeric protein into condensate droplets and of the …

Heterotypic electrostatic interactions control complex phase separation of tau and prion into multiphasic condensates and co-aggregates

SK Rai, R Khanna, A Avni… - Proceedings of the …, 2023 - National Acad Sciences
Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to perform a wide range of critical cellular functions by maintaining spatiotemporal …

Biomolecular condensates modulate membrane lipid packing and hydration

A Mangiarotti, M Siri, NW Tam, Z Zhao… - Nature …, 2023 - nature.com
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in
cell biology, playing an important role in a diverse range of processes across different …

Substitution of Met-38 to Ile in γ-synuclein found in two patients with amyotrophic lateral sclerosis induces aggregation into amyloid

LD Aubrey, N Ninkina, SM Ulamec… - Proceedings of the …, 2024 - National Acad Sciences
α-, β-, and γ-Synuclein are intrinsically disordered proteins implicated in physiological
processes in the nervous system of vertebrates. α-synuclein (αSyn) is the amyloidogenic …

The bacterial nucleoid-associated proteins, HU and Dps, condense DNA into context-dependent biphasic or multiphasic complex coacervates

A Gupta, A Joshi, K Arora, S Mukhopadhyay… - Journal of Biological …, 2023 - ASBMB
The bacterial chromosome, known as its nucleoid, is an amorphous assemblage of globular
nucleoprotein domains. It exists in a state of phase separation from the cell's cytoplasm, as …