Evolution and Developmental System Drift in the Endoderm Gene Regulatory Network of Caenorhabditis and Other Nematodes

CK Ewe, YN Torres Cleuren… - Frontiers in Cell and …, 2020 - frontiersin.org
Developmental gene regulatory networks (GRNs) underpin metazoan embryogenesis and
have undergone substantial modification to generate the tremendous variety of animal forms …

Single worm transcriptomics identifies a developmental core network of oscillating genes with deep conservation across nematodes

S Sun, C Rödelsperger, RJ Sommer - Genome research, 2021 - genome.cshlp.org
High-resolution spatial and temporal maps of gene expression have facilitated a
comprehensive understanding of animal development and evolution. In nematodes, the …

Evolutionary Change in Gut Specification in Caenorhabditis Centers on the GATA Factor ELT-3 in an Example of Developmental System Drift

G Broitman-Maduro, MF Maduro - Journal of Developmental Biology, 2023 - mdpi.com
Cells in a developing animal embryo become specified by the activation of cell-type-specific
gene regulatory networks. The network that specifies the gut in the nematode …

Adapting the smart-seq2 protocol for robust single worm RNA-seq

L Serra, DZ Chang, M Macchietto, K Williams… - Bio …, 2018 - bio-protocol.org
Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols
without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 …

Using single-worm RNA sequencing to study C. elegans responses to pathogen infection

AJ Wang, P Wibisono, BM Geppert, Y Liu - BMC genomics, 2022 - Springer
Background Caenorhabditis elegans is an excellent research model whose populations
have been used in many studies to address various biological questions. Although worm-to …

High-resolution transcriptome datasets during embryogenesis of plant-parasitic nematodes

X Wang, Z Guo, D Dai, C Xie, Z Zhao, J Zheng, M Sun… - Scientific Data, 2024 - nature.com
Understanding the transcriptional regulatory characteristics throughout the embryogenesis
of plant-parasitic nematodes is crucial for elucidating their developmental processes' …

Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome

L Serra, M Macchietto, A Macias-Muñoz… - G3: Genes …, 2019 - academic.oup.com
Entomopathogenic nematodes from the genus Steinernema are lethal insect parasites that
quickly kill their insect hosts with the help of their symbiotic bacteria. Steinernema …

The gene regulatory program of Acrobeloides nanus reveals conservation of phylum-specific expression

PH Schiffer, AL Polsky, AG Cole… - Proceedings of the …, 2018 - National Acad Sciences
The evolution of development has been studied through the lens of gene regulation by
examining either closely related species or extremely distant animals of different phyla. In …

A revised adaptation of the smart-Seq2 protocol for single-nematode RNA-seq

D Chang, L Serra, D Lu, A Mortazavi… - RNA Abundance Analysis …, 2021 - Springer
The advancement of transcriptomic studies in plant parasitic nematodes will greatly benefit
from the development of single-nematode RNA-seq methods. Since many plant parasitic …

The GATA factor ELT-3 specifies endoderm in Caenorhabditis angaria in an ancestral gene network

G Broitman-Maduro, S Sun, T Kikuchi… - …, 2022 - journals.biologists.com
Endoderm specification in Caenorhabditis elegans occurs through a network in which
maternally provided SKN-1/Nrf, with additional input from POP-1/TCF, activates the GATA …