Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Particle tracking is of key importance for quantitative analysis of intracellular dynamic
processes from time-lapse microscopy image data. Because manually detecting and …

With the rapid development of optical microscopic techniques, explorations on the chemical
and biological properties of target objects in biological samples at single-molecule/particle …

Genetic studies have suggested a functional link between cholesterol/sphingolipid
metabolism and endocytic membrane traffic. Here we show that perturbing the cholesterol …

Tracking subcellular structures as well as viral structures displayed asparticles' in
fluorescence microscopy images yields quantitative information on the underlying dynamical …

In order to quantitatively analyze biological images and study underlying mechanisms of the
cellular and subcellular processes, it is often required to track a large number of particles …

As camera pixel arrays have grown larger and faster, and optical microscopy techniques
ever more refined, there has been an explosion in the quantity of data acquired during …

Recent advances in optical microscopy have enabled the acquisition of very large datasets
from living cells with unprecedented spatial and temporal resolutions. Our ability to process …

Time-lapse microscopy is an important technique to study the dynamics of various biological
processes. The labor-intensive manual analysis of microscopy videos is increasingly …

Assessing dynamic processes at single molecule scales is key toward capturing life at the
level of its molecular actors. Widefield superresolution methods, such as STORM, PALM …