EGF, a veteran of wound healing: highlights on its mode of action, clinical applications with focus on wound treatment, and recent drug delivery strategies

K Shakhakarmi, JE Seo, S Lamichhane… - Archives of Pharmacal …, 2023 - Springer
Epidermal growth factor (EGF) has been used in wound management and regenerative
medicine since the late 1980s. It has been widely utilized for a long time and still is because …

Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

A Eskandari, NG Nezhad, TC Leow… - Archives of …, 2024 - Springer
Producing recombinant proteins is a major accomplishment of biotechnology in the past
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …

Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm

M Li, Z Wang, M Zhou, C Zhang, K Zhi, S Liu… - Frontiers in …, 2022 - frontiersin.org
Increasing demand for recombinant proteins necessitates efficient protein production
processes. In this study, a continuous process for human epidermal growth factor (hEGF) …

Cationic Solid SMEDDS of Efavirenz for Improved Oral Delivery: Development by Central Composite Design, In Vitro and In Vivo Evaluation

SK Thota, N Dudhipala, V Katla, K Veerabrahma - AAPS PharmSciTech, 2023 - Springer
Efavirenz (EFV) is an anti-HIV drug with high dose and 40% oral bioavailability (BA). The
aim was to improve the bioavailability by designing cationic solid SMEDDS. Solubility data …

Refolding of bioactive human epidermal growth factor from E. coli BL21 (DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity

IP Maksum, Y Yosua, A Nabiel, RD Pratiwi… - Heliyon, 2022 - cell.com
Human epidermal growth factor (hEGF) is a mitogenic protein widely used in pharmaceutical
and cosmetic industries, thus recombinant DNA technology has been applied to meet the …

Production of recombinant human epidermal growth factor in Escherichia coli: Strategic upstream and downstream considerations for high protein yield

RS Wong, MWO Liew, EBB Ong - Process Biochemistry, 2024 - Elsevier
The human epidermal growth factor (hEGF) is a powerful mitogen used in medical treatment
and cosmetics. However, the recovery of hEGF from natural sources is insufficient to meet …

The effects of combined co-expression of groel/es and trigger factor chaperone towards Thermus thermophilus DNA polymerase enzyme expression on Escherichia …

IP Maksum, DF Utami, S Sriwidodo… - Journal of Applied …, 2024 - japsonline.com
The Thermus thermophilus (Tth) DNA polymerase enzyme, originating from the thermophilic
bacterium T. thermophilus, exhibits bifunctionally as both a DNA polymerase and a reverse …

Overexpression of soluble recombinant Thermus thermophilus (Tth) DNA polymerase in Escherichia coli BL21 (DE3) using an MBP fusion tag as a solubility enhancer

IP Maksum, DF Utami, EA Nurhakim… - Journal of Applied …, 2022 - japsonline.com
Tth DNA polymerase is a thermostable enzyme derived from Thermus thermophilus and acts
as a DNA polymerase and reverse transcriptase. Escherichia coli is used for large-scale …

An overview of culture conditions for recombinant protein expression in Escherichia coli

IP Maksum, FPU Latifah, A Nabiel… - Journal of Applied …, 2023 - biotechrep.ir
Escherichia coli is one of the most commonly used organisms for producing recombinant
protein. The recognized cell genome and well-known cell factory of the bacteria make it an …

Stabilization of eye drops containing autologous serum and recombinant human epidermal growth factor for dry eye syndrome

S Sriwidodo, ISK Syah, IP Maksum… - Journal of Advanced …, 2020 - journals.lww.com
Human epidermal growth factor (hEGF) and autologous serum are considered safer and
more effective in treating dry eye syndrome. However, suitable formulas and preparation …