Learning optimal wavefront shaping for multi-channel imaging
Fast acquisition of depth information is crucial for accurate 3D tracking of moving objects.
Snapshot depth sensing can be achieved by wavefront coding, in which the point-spread …
Snapshot depth sensing can be achieved by wavefront coding, in which the point-spread …
Long-wavelength traveling waves of vasomotion modulate the perfusion of cortex
Brain arterioles are active, multicellular complexes whose diameters oscillate at∼ 0.1 Hz.
We assess the physiological impact and spatiotemporal dynamics of vaso-oscillations in the …
We assess the physiological impact and spatiotemporal dynamics of vaso-oscillations in the …
Systematic and quantitative comparison of lattice and Gaussian light-sheets
The axial resolving power of a light-sheet microscope is determined by the thickness of the
illumination beam and the numerical aperture of its detection optics. Bessel-beam based …
illumination beam and the numerical aperture of its detection optics. Bessel-beam based …
High-throughput volumetric mapping of synaptic transmission
Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal
resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed …
resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed …
Depth-enhanced high-throughput microscopy by compact PSF engineering
N Opatovski, E Nehme, N Zoref, I Barzilai… - Nature …, 2024 - nature.com
High-throughput microscopy is vital for screening applications, where three-dimensional
(3D) cellular models play a key role. However, due to defocus susceptibility, current 3D high …
(3D) cellular models play a key role. However, due to defocus susceptibility, current 3D high …
Effects of coherence on temporal resolution
Measuring small separations between two optical sources, either in space or in time,
constitutes an important metrological challenge as standard intensity-only measurements …
constitutes an important metrological challenge as standard intensity-only measurements …
Bessel-droplet foci enable high-resolution and high-contrast volumetric imaging of synapses and circulation in the brain in vivo
Bessel beam has long been utilized in physics for its ability to maintain lateral confinement
during propagation. When used for two-photon fluorescence microscopy, Bessel foci have …
during propagation. When used for two-photon fluorescence microscopy, Bessel foci have …
Dynamic volumetric imaging of mouse cerebral blood vessels in vivo with an ultralong anti-diffracting beam
Y Guo, L Wang, Z Luo, Y Zhu, X Gao, X Weng, Y Wang… - Molecules, 2023 - mdpi.com
Volumetric imaging of a mouse brain in vivo with one-photon and two-photon ultralong anti-
diffracting (UAD) beam illumination was performed. The three-dimensional (3D) structure of …
diffracting (UAD) beam illumination was performed. The three-dimensional (3D) structure of …
[HTML][HTML] Extended depth-of-field light-sheet microscopy improves imaging of large volumes at high numerical aperture
Light-sheet microscopes must compromise among field of view, optical sectioning,
resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses …
resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses …
Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging
PY Lin, SPL Hwang, CH Lee… - Journal of Biomedical …, 2021 - spiedigitallibrary.org
Significance: Two-photon microscopy has become the standard platform for deep-tissue
fluorescence imaging. However, the use of point scanning in conventional two-photon …
fluorescence imaging. However, the use of point scanning in conventional two-photon …