Biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening for over-production phenotypes
With rapid advances in the development of metabolic pathways and synthetic biology
toolkits, a persisting challenge in microbial bioproduction is how to optimally rewire …
toolkits, a persisting challenge in microbial bioproduction is how to optimally rewire …
Harnessing non-Watson–Crick's base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cells
Genomic DNA of the cyanophage S-2L virus is composed of 2-aminoadenine (Z), thymine
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …
Improvement of floricultural traits in ornamental crops using genome editing tools
Floriculture is one of the most promising sectors of the horticulture industry with immense
aesthetic, social, medicinal and economic value. Ornamentals are rich in natural pigments …
aesthetic, social, medicinal and economic value. Ornamentals are rich in natural pigments …
Exploring and engineering PAM-diverse Streptococci Cas9 for PAM-directed bifunctional and titratable gene control in bacteria
The RNA-guided Cas9s serve as powerful tools for programmable gene editing and
regulation; their targeting scopes and efficacies, however, are always constrained by the …
regulation; their targeting scopes and efficacies, however, are always constrained by the …
Establishing an Autonomous Cascaded Artificial Dynamic (AutoCAD) regulation system for improved pathway performance
Endogenous metabolic pathways in microbial cells are usually precisely controlled by
sophisticated regulation networks. However, the lack of such regulations when introducing …
sophisticated regulation networks. However, the lack of such regulations when introducing …
[HTML][HTML] Engineering Cas9: next generation of genomic editors
MA Kovalev, AI Davletshin, DS Karpov - Applied Microbiology and …, 2024 - Springer
The Cas9 endonuclease of the CRISPR/Cas type IIA system from Streptococcus pyogenes
is the heart of genome editing technology that can be used to treat human genetic and viral …
is the heart of genome editing technology that can be used to treat human genetic and viral …
CRISPRactivation-SMS, a message for PAM sequence independent gene up-regulation in Escherichia coli
M Klanschnig, M Cserjan-Puschmann… - Nucleic Acids …, 2022 - academic.oup.com
Governance of the endogenous gene regulatory network enables the navigation of cells
towards beneficial traits for recombinant protein production. CRISPRactivation and …
towards beneficial traits for recombinant protein production. CRISPRactivation and …
Expanding the scope of bacterial CRISPR activation with PAM-flexible dCas9 variants
CRISPR-Cas transcriptional tools have been widely applied for programmable regulation of
complex biological networks. In comparison to eukaryotic systems, bacterial CRISPR …
complex biological networks. In comparison to eukaryotic systems, bacterial CRISPR …
In vivo base editing rescues primary hyperoxaluria type 1 in rats
Z Chen, D Zhang, R Zheng, L Yang, Y Huo, D Zhang… - Kidney International, 2024 - Elsevier
Primary hyperoxaluria type 1 (PH1) is a childhood-onset autosomal recessive disease,
characterized by nephrocalcinosis, multiple recurrent urinary calcium oxalate stones, and a …
characterized by nephrocalcinosis, multiple recurrent urinary calcium oxalate stones, and a …
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways
Microbial synthesis has emerged as a promising and sustainable alternative to traditional
chemical synthesis and plant extraction. However, the competition between synthetic …
chemical synthesis and plant extraction. However, the competition between synthetic …