Identifying the interactions established by a protein of interest can be a critical step in
understanding its function. This is especially true when an unknown protein of interest is …

TAP (tandem affinity purification) allows rapid and clean isolation of a tagged protein along
with its interacting partners from cell lysates. Initially developed in yeast, the TAP method …

A GGGGCC hexanucleotide repeat expansion within the C9orf72 gene is the most common
genetic cause of both amyotrophic lateral sclerosis and frontotemporal dementia. Sense and …

Tandem affinity purification coupled to mass spectrometry (TAP-MS) is one of the most
advanced methods to characterize protein complexes in plants, giving a comprehensive …

The Hippo pathway controls metazoan organ growth by regulating cell proliferation and
apoptosis. Many components have been identified, but our knowledge of the composition …

Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes
from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of …

Perturbation of huntingtin (HTT)'s physiological function is one postulated pathogenic factor
in Huntington's disease (HD). However, little is known how HTT is regulated in vivo. In a …

Abstract The atypical cadherins Fat (Ft) and Dachsous (Ds) control tissue growth through the
Salvador–Warts–Hippo (SWH) pathway, and also regulate planar cell polarity and …

The Hippo pathway is a conserved signaling cascade that modulates tissue growth.
Although its core elements are well defined, factors modulating Hippo transcriptional outputs …

Adult stem cells coordinate intrinsic and extrinsic, local and systemic, cues to maintain the
proper balance between self-renewal and differentiation. However, the precise mechanisms …