[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Potential of different enzyme immobilization strategies to improve enzyme performance

C Garcia‐Galan, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Enzyme biocatalysis plays a very relevant role in the development of many chemical
industries, eg, energy, food or fine chemistry. To achieve this goal, enzyme immobilization is …

Improvement of enzyme activity, stability and selectivity via immobilization techniques

C Mateo, JM Palomo, G Fernandez-Lorente… - Enzyme and microbial …, 2007 - Elsevier
In spite of their excellent catalytic properties, enzyme properties have to be usually improved
before their implementation at industrial scale (where many cycles of high yield processes …

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

R Fernandez-Lafuente - Enzyme and microbial technology, 2009 - Elsevier
The moderate stability of enzymes is one of the main drawbacks that hinder general
implementation of these interesting biocatalysts at industrial scale. An especially complex …

Immobilised enzymes: science or art?

L Cao - Current opinion in chemical biology, 2005 - Elsevier
Enzyme immobilisation is experiencing an important transition. Combinatorial approaches
are increasingly applied in the design of robust immobilised enzymes by rational …

Enzymes on material surfaces

JN Talbert, JM Goddard - Colloids and Surfaces B: Biointerfaces, 2012 - Elsevier
Enzyme interactions with material surfaces are of interest for industrial food and
pharmaceutical transformations, biosensors, artificial cells, cell free reactions, drug and …

Bipolar electrochemical mechanism for the propulsion of catalytic nanomotors in hydrogen peroxide solutions

Y Wang, RM Hernandez, DJ Bartlett, JM Bingham… - Langmuir, 2006 - ACS Publications
Bimetallic nanorods are propelled in aqueous solutions by the catalytic decomposition of
hydrogen peroxide to oxygen and water. Several mechanisms (interfacial tension gradients …

Twin-engine Janus supramolecular nanomotors with counterbalanced motion

J Shao, S Cao, H Che, MT De Martino… - Journal of the …, 2022 - ACS Publications
Supramolecular nanomotors were created with two types of propelling forces that were able
to counterbalance each other. The particles were based on bowl-shaped polymer vesicles …

Coupling chemical modification and immobilization to improve the catalytic performance of enzymes

RC Rodrigues, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Chemical modification and immobilization of enzymes have been usually considered
unrelated tools to improve biocatalyst features. However, there are many examples where a …

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

DA Cowan, R Fernandez-Lafuente - Enzyme and Microbial Technology, 2011 - Elsevier
The immobilization of proteins (mostly typically enzymes) onto solid supports is mature
technology and has been used successfully to enhance biocatalytic processes in a wide …