[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria

DC Volke, E Orsi, PI Nikel - Current Opinion in Microbiology, 2023 - Elsevier
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Stepping on the gas to a circular economy: accelerating development of carbon-negative chemical production from gas fermentation

N Fackler, BD Heijstra, BJ Rasor… - Annual review of …, 2021 - annualreviews.org
Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change
poses significant environmental, economic, and social challenges globally. Technologies …

Pollution to products: recycling of 'above ground'carbon by gas fermentation

M Köpke, SD Simpson - Current Opinion in Biotechnology, 2020 - Elsevier
Climate crisis and rapid population growth are posing some of the most urgent challenges to
mankind and have intensified the need for the deployment of carbon recycling and carbon …

Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators

X Dong, BGH Guthrie, M Alexander, C Noecker… - Nature …, 2022 - nature.com
Eggerthella lenta is a prevalent human gut Actinobacterium implicated in drug, dietary
phytochemical, and bile acid metabolism and associated with multiple human diseases. No …

Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy

M Pavan, K Reinmets, S Garg, AP Mueller… - Metabolic …, 2022 - Elsevier
High levels of anthropogenic CO 2 emissions are driving the warming of global climate. If
this pattern of increasing emissions does not change, it will cause further climate change …

Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum

J Shin, J Bae, H Lee, S Kang, S Jin… - Proceedings of the …, 2023 - National Acad Sciences
Acetogenic bacteria are a unique biocatalyst that highly promises to develop the sustainable
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …

Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium …

M Flaiz, G Ludwig, FR Bengelsdorf, P Dürre - Biotechnology for Biofuels, 2021 - Springer
Background The interest in using methanol as a substrate to cultivate acetogens increased
in recent years since it can be sustainably produced from syngas and has the additional …

Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms

B Bourgade, NP Minton, MA Islam - FEMS Microbiology …, 2021 - academic.oup.com
Unabated mining and utilisation of petroleum and petroleum resources and their conversion
to essential fuels and chemicals have drastic environmental consequences, contributing to …

Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol

PA Sanford, BM Woolston - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•Synthetic methylotrophy achieved in E. coli via ribulose monophosphate
cycle.•Synthetic methylotrophy has been achieved in E. coli via reductive glycine …

Synthetic biology on acetogenic bacteria for highly efficient conversion of C1 gases to biochemicals

S Jin, J Bae, Y Song, N Pearcy, J Shin, S Kang… - International journal of …, 2020 - mdpi.com
Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of
C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen …