Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Sample preparation is one of the most important steps in metabolome analysis. The
challenges of determining microbial metabolome have been well discussed within the …

The methylotrophic yeast Pichia pastoris (aka Komagataella phaffii) is one of the most
commonly used hosts for industrial production of recombinant proteins. As a non …

The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

The use of the methylotrophic yeast Pichia pastoris (Komagataella phaffi) to produce
heterologous proteins has been largely reported. However, investigations addressing the …

Abstract β-Alanine (3-aminopropionic acid) is the only naturally occurring β-amino acid and
an important precursor for the synthesis of a variety of nitrogen-containing chemicals …

Quantitative metabolic profiling is preceded by dedicated sample preparation protocols.
These multistep procedures require detailed optimization and thorough validation. In this …

History of metabolism originates with yeast making bread. In fact, study based on “Yeast”
was so crucial in the development of the field of biochemistry that the word “enzyme” is …

A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on
cold aqueous methanol quenching was re-evaluated and optimized to reduce metabolite …