Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants

EM Temsch, P Koutecký, T Urfus, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
The estimation of nuclear DNA content has been by far the most popular application of flow
cytometry in plants. Because flow cytometry measures relative fluorescence intensities of …

Application‐based guidelines for best practices in plant flow cytometry

E Sliwinska, J Loureiro, IJ Leitch, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
Flow cytometry (FCM) is currently the most widely‐used method to establish nuclear DNA
content in plants. Since simple, 1‐3‐parameter, flow cytometers, which are sufficient for most …

Novel approaches for species concepts and delimitation in polyploids and hybrids

E Hörandl - Plants, 2022 - mdpi.com
Hybridization and polyploidization are important processes for plant evolution. However,
classification of hybrid or polyploid species has been notoriously difficult because of the …

Application of Single-Cell Assay for Transposase-Accessible Chromatin with High Throughput Sequencing in Plant Science: Advances, Technical Challenges, and …

C Lu, Y Wei, M Abbas, H Agula, E Wang… - International Journal of …, 2024 - mdpi.com
The Single-cell Assay for Transposase-Accessible Chromatin with high throughput
sequencing (scATAC-seq) has gained increasing popularity in recent years, allowing for …

Best practices in the flow cytometry of microalgae

D Čertnerová, DW Galbraith - Cytometry Part A, 2021 - Wiley Online Library
Microalgae are photosynthetic microorganisms with a major influence on global ecosystems.
Further, owing to the production of various secondary metabolites, microalgae are also …

Plant material selection, collection, preservation, and storage for nuclear DNA content estimation

M Čertner, M Lučanová, E Sliwinska, F Kolář… - Cytometry Part …, 2022 - Wiley Online Library
In theory, any plant tissue providing intact nuclei in sufficient quantity is suitable for nuclear
DNA content estimation using flow cytometry (FCM). While this certainly opens a wide …

The use of flow cytometry for estimating genome sizes and DNA ploidy levels in plants

J Loureiro, M Čertner, M Lučanová, E Sliwinska… - Plant Cytogenetics and …, 2023 - Springer
Flow cytometry has emerged as a uniquely flexible, accurate, and widely applicable
technology for the analysis of plant cells. One of its most important applications centers on …

Best practices for instrument settings and raw data analysis in plant flow cytometry

P Koutecký, T Smith, J Loureiro, P Kron - Cytometry Part A, 2023 - Wiley Online Library
Flow cytometry (FCM) is now the most widely used method to determine ploidy levels and
genome size of plants. To get reliable estimates and allow reproducibility of measurements …

Mastering DNA content estimation by flow cytometry as an efficient tool for plant breeding and biodiversity research

M Fomicheva, E Domblides - Methods and Protocols, 2023 - mdpi.com
Flow cytometry gives a unique opportunity to analyze thousands of individual cells for
multiple parameters in a course of minutes. The most commonly used flow cytometry …

Phylogenetic, cytogenetic and morphological evidences are critical for recognizing a new genus: Valdesiana, an Iberian intergeneric allopolyploid between Schenkia …

Z Díaz Lifante, M Escudero, C Andrés Camacho… - Plant Systematics and …, 2023 - Springer
The present taxonomic status of Schenkia elegans, endemism recently described in the
Iberian Peninsula, and its relationship with the sympatric and the nearest morphological …