The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
CRISPR-Cas9 system: A new-fangled dawn in gene editing
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes
Some pathogens and pests deliver small RNAs (sRNAs) into host cells to suppress host
immunity. Conversely, hosts also transfer sRNAs into pathogens and pests to inhibit their …
immunity. Conversely, hosts also transfer sRNAs into pathogens and pests to inhibit their …
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Z Shimatani, S Kashojiya, M Takayama, R Terada… - Nature …, 2017 - nature.com
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-
AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in …
AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in …
The synaptonemal complex imposes crossover interference and heterochiasmy in Arabidopsis
L Capilla-Pérez, S Durand, A Hurel… - Proceedings of the …, 2021 - National Acad Sciences
Meiotic crossovers (COs) have intriguing patterning properties, including CO interference,
the tendency of COs to be well-spaced along chromosomes, and heterochiasmy, the marked …
the tendency of COs to be well-spaced along chromosomes, and heterochiasmy, the marked …
A multipurpose toolkit to enable advanced genome engineering in plants
T Čermák, SJ Curtin, J Gil-Humanes, R Čegan… - The Plant …, 2017 - academic.oup.com
We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …
and dicot genomes using a variety of genome engineering approaches. Our reagents …
A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants
CRISPR/Cas9 genome targeting systems have been applied to a variety of species.
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
A PARTHENOGENESIS allele from apomictic dandelion can induce egg cell division without fertilization in lettuce
CJ Underwood, K Vijverberg, D Rigola, S Okamoto… - Nature Genetics, 2022 - nature.com
Apomixis, the clonal formation of seeds, is a rare yet widely distributed trait in flowering
plants. We have isolated the PARTHENOGENESIS (PAR) gene from apomictic dandelion …
plants. We have isolated the PARTHENOGENESIS (PAR) gene from apomictic dandelion …
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
ZP Wang, HL Xing, L Dong, HY Zhang, CY Han… - Genome biology, 2015 - Springer
Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome
editing system are usually mosaics in the T1 generation. In this study, we used egg cell …
editing system are usually mosaics in the T1 generation. In this study, we used egg cell …
A CRISPR/Cas9 toolkit for multiplex genome editing in plants
HL Xing, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …