The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …

Some pathogens and pests deliver small RNAs (sRNAs) into host cells to suppress host
immunity. Conversely, hosts also transfer sRNAs into pathogens and pests to inhibit their …

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-
AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in …

Meiotic crossovers (COs) have intriguing patterning properties, including CO interference,
the tendency of COs to be well-spaced along chromosomes, and heterochiasmy, the marked …

We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …

CRISPR/Cas9 genome targeting systems have been applied to a variety of species.
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …

Apomixis, the clonal formation of seeds, is a rare yet widely distributed trait in flowering
plants. We have isolated the PARTHENOGENESIS (PAR) gene from apomictic dandelion …

Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome
editing system are usually mosaics in the T1 generation. In this study, we used egg cell …

Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …