Research progress on the functions and biosynthesis of theaflavins
Y Liu, D Wang, J Li, Z Zhang, Y Wang, C Qiu, Y Sun… - Food Chemistry, 2024 - Elsevier
Theaflavins are beneficial to human health due to various bioactivities. Biosynthesis of
theaflavins using polyphenol oxidase (PPO) is advantageous due to cost effectiveness and …
theaflavins using polyphenol oxidase (PPO) is advantageous due to cost effectiveness and …
Reshaping substrate-binding pocket of leucine dehydrogenase for bidirectionally accessing structurally diverse substrates
T Wu, Y Wang, N Zhang, D Yin, Y Xu, Y Nie, X Mu - ACS Catalysis, 2022 - ACS Publications
Steric hindrance modification-based pocket reshaping is an effective approach for enzyme–
substrate acceptance evolution. However, previous studies were limited to using the …
substrate acceptance evolution. However, previous studies were limited to using the …
Biosynthesis of l-alanine from cis-butenedioic anhydride catalyzed by a triple-enzyme cascade via a genetically modified strain
R Cui, Z Liu, P Yu, L Zhou, Z Zhou - Green Chemistry, 2021 - pubs.rsc.org
In industry, L-alanine is biosynthesized using fermentation methods or catalyzed from L-
aspartic acid by aspartate β-decarboxylase (ASD). In this study, a triple-enzyme system was …
aspartic acid by aspartate β-decarboxylase (ASD). In this study, a triple-enzyme system was …
Mining and engineering of valine dehydrogenases from a hot spring sediment metagenome for the synthesis of chiral non-natural L-amino acids
X Yin, W Gong, Z Zhan, W Wei, M Li, J Jiao, B Chen… - Molecular …, 2022 - Elsevier
Valine dehydrogenases (ValDHs) represent promising candidates for the development of
biocatalysts for short chain amino acids or amines synthesis. However, only a limited …
biocatalysts for short chain amino acids or amines synthesis. However, only a limited …
The identification of a robust leucine dehydrogenase from a directed soil metagenome for efficient synthesis of L‐2‐aminobutyric acid
Y Liu, X Zhong, Z Luo, X Meng, R Li… - Biotechnology …, 2023 - Wiley Online Library
Abstract L‐2‐aminobutyric acid (L‐2‐ABA) is a chiral precursor for the synthesis of anti‐
epileptic drug levetiracetam and anti‐tuberculosis drug ethambutol. Asymmetric synthesis of …
epileptic drug levetiracetam and anti‐tuberculosis drug ethambutol. Asymmetric synthesis of …
Rapid screening of point mutations by mismatch amplification mutation assay PCR
F Zhang, ZY Liu, S Liu, WG Zhang, BB Wang… - Applied Microbiology …, 2024 - Springer
Metabolic engineering frequently makes use of point mutation and saturation mutation
library creation. At present, sequencing is the only reliable and direct technique to detect …
library creation. At present, sequencing is the only reliable and direct technique to detect …
Improving the activity and synergistic catalysis of L-aspartate β-decarboxylase by arginine introduction on the surface
M Hao, R Cui, X Zhu, L Han, Z Zhou… - Catalysis Science & …, 2022 - pubs.rsc.org
Decarboxylase typically catalyzes reactions under acidic conditions, which is the drawback
for its synergistic catalysis with other enzymes because most enzymes catalyze reactions …
for its synergistic catalysis with other enzymes because most enzymes catalyze reactions …
Identification of key residues for the activity of aspartate 4-decarboxylase towards l-3-methylaspartate
M Hao, L Han, Z Zhou, Z Liu - Systems Microbiology and …, 2023 - Springer
Abstract Aspartate 4-decarboxylase (ASD) has been modified to obtain the catalytic ability of
the unnatural substrate l-3-methylaspartate. However, the mechanism remains to be …
the unnatural substrate l-3-methylaspartate. However, the mechanism remains to be …
Isolation and mechanism analysis of a catalytic efficiency improved L-aspartate β-decarboxylase toward 3-methylaspartic acid
Y Liu, M Hao, Z Zhou, Z Liu - Systems Microbiology and Biomanufacturing, 2022 - Springer
L-Aspartate β-decarboxylase from Acinetobacter radioresistens (Ar ASD) has been modified
to convert 3-methylaspartic acid into 2-aminobutyric acid, which activated a novel process …
to convert 3-methylaspartic acid into 2-aminobutyric acid, which activated a novel process …
Double enzyme tandem preparation method of l-2-aminobutyric acid
Z Zhou, Z Liu, Y Liu, L Zhou, W Cui… - US Patent …, 2024 - Google Patents
Disclosed is a double enzyme tandem preparation method of L-2-aminobutyric acid, and
belongs to the field of bioengineering. In the disclosure, recombinant Escherichia coli …
belongs to the field of bioengineering. In the disclosure, recombinant Escherichia coli …