The biology of the HIV-1 latent reservoir and implications for cure strategies

LB Cohn, N Chomont, SG Deeks - Cell host & microbe, 2020 - cell.com
Antiretroviral therapy (ART) inhibits HIV replication but is not curative. During ART, the
integrated HIV genome persists indefinitely within CD4+ T cells and perhaps other cells …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2021 - Wiley Online Library
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

Deep immune profiling of MIS-C demonstrates marked but transient immune activation compared with adult and pediatric COVID-19

LA Vella, JR Giles, AE Baxter, DA Oldridge… - Science …, 2021 - science.org
Pediatric coronavirus disease 2019 (COVID-19) after SARS-CoV-2 infection is associated
with fewer hospitalizations and often milder disease than in adults. A subset of children …

[HTML][HTML] Single-cell multiomics reveals persistence of HIV-1 in expanded cytotoxic T cell clones

JA Collora, R Liu, D Pinto-Santini, N Ravindra… - Immunity, 2022 - cell.com
Understanding the drivers and markers of clonally expanding HIV-1-infected CD4+ T cells is
essential for HIV-1 eradication. We used single-cell ECCITE-seq, which captures surface …

Allelic variation in class I HLA determines CD8+ T cell repertoire shape and cross-reactive memory responses to SARS-CoV-2

JM Francis, D Leistritz-Edwards, A Dunn, C Tarr… - Science …, 2021 - science.org
Effective presentation of antigens by human leukocyte antigen (HLA) class I molecules to
CD8+ T cells is required for viral elimination and generation of long-term immunological …

Why and where an HIV cure is needed and how it might be achieved

T Ndung'u, JM McCune, SG Deeks - Nature, 2019 - nature.com
Despite considerable global investment, only 60% of people who live with HIV currently
receive antiretroviral therapy. The sustainability of current programmes remains unknown …

Integrated single-cell analysis of multicellular immune dynamics during hyperacute HIV-1 infection

SW Kazer, TP Aicher, DM Muema, SL Carroll… - Nature medicine, 2020 - nature.com
Cellular immunity is critical for controlling intracellular pathogens, but individual cellular
dynamics and cell–cell cooperativity in evolving human immune responses remain poorly …

Single-cell transcriptomics identifies prothymosin α restriction of HIV-1 in vivo

A Geretz, PK Ehrenberg, RJ Clifford… - Science Translational …, 2023 - science.org
Host restriction factors play key roles in innate antiviral defense, but it remains poorly
understood which of them restricts HIV-1 in vivo. Here, we used single-cell transcriptomic …

Block-and-lock: new horizons for a cure for HIV-1

I Moranguinho, ST Valente - Viruses, 2020 - mdpi.com
HIV-1/AIDS remains a global public health problem. The world health organization (WHO)
reported at the end of 2019 that 38 million people were living with HIV-1 worldwide, of which …

The forces driving clonal expansion of the HIV-1 latent reservoir

R Liu, FR Simonetti, YC Ho - Virology journal, 2020 - Springer
Despite antiretroviral therapy (ART) which halts HIV-1 replication and reduces plasma viral
load to clinically undetectable levels, viral rebound inevitably occurs once ART is …