Climate change opens new frontiers for marine species in the Arctic: Current trends and future invasion risks
FT Chan, K Stanislawczyk, AC Sneekes… - Global change …, 2019 - Wiley Online Library
Climate change and increased anthropogenic activities are expected to elevate the potential
of introducing nonindigenous species (NIS) into the Arctic. Yet, the knowledge base needed …
of introducing nonindigenous species (NIS) into the Arctic. Yet, the knowledge base needed …
Advantages and limitations of environmental DNA/RNA tools for marine biosecurity: management and surveillance of non-indigenous species
To enable successful management of marine bioinvasions, timely and robust scientific
advice is required. This knowledge should inform managers and stakeholders on the …
advice is required. This knowledge should inform managers and stakeholders on the …
Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter
J Spens, AR Evans, D Halfmaerten… - Methods in Ecology …, 2017 - Wiley Online Library
Aqueous environmental DNA (eDNA) is an emerging efficient non‐invasive tool for species
inventory studies. To maximize performance of downstream quantitative PCR (qPCR) and …
inventory studies. To maximize performance of downstream quantitative PCR (qPCR) and …
Particle size distribution and optimal capture of aqueous macrobial eDNA
Using environmental DNA (eDNA) to detect aquatic macroorganisms is a new survey
method with broad applicability. However, the origin, state and fate of aqueous macrobial …
method with broad applicability. However, the origin, state and fate of aqueous macrobial …
Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding
To manage and conserve biodiversity, one must know what is being lost, where, and why, as
well as which remedies are likely to be most effective. Metabarcoding technology can …
well as which remedies are likely to be most effective. Metabarcoding technology can …
A comparison of droplet digital polymerase chain reaction (PCR), quantitative PCR and metabarcoding for species‐specific detection in environmental DNA
Targeted species‐specific and community‐wide molecular diagnostics tools are being used
with increasing frequency to detect invasive or rare species. Few studies have compared the …
with increasing frequency to detect invasive or rare species. Few studies have compared the …
Wanted dead or alive? Using metabarcoding of environmental DNA and RNA to distinguish living assemblages for biosecurity applications
High-throughput sequencing metabarcoding studies in marine biosecurity have largely
focused on targeting environmental DNA (eDNA). DNA can persist extracellularly in the …
focused on targeting environmental DNA (eDNA). DNA can persist extracellularly in the …
Detection of introduced and resident marine species using environmental DNA metabarcoding of sediment and water
Environmental DNA (eDNA) surveys are increasingly being used for biodiversity monitoring,
principally because they are sensitive and can provide high resolution community …
principally because they are sensitive and can provide high resolution community …
Accurate multiplexing and filtering for high-throughput amplicon-sequencing
Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …
Scraping the bottom of the barrel: are rare high throughput sequences artifacts?
Metabarcoding data generated using next-generation sequencing (NGS) technologies are
overwhelmed with rare taxa and skewed in Operational Taxonomic Unit (OTU) frequencies …
overwhelmed with rare taxa and skewed in Operational Taxonomic Unit (OTU) frequencies …