The Hippo pathway is an evolutionarily conserved regulator of tissue growth that integrates
inputs from both polarity and actomyosin networks. An upstream activator of the Hippo …

Mechanical forces are critical but poorly understood inputs for organogenesis and wound
healing. Calcium ions (Ca 2+) are critical second messengers in cells for integrating …

Time-lapse imaging is an essential tool to study dynamic biological processes that cannot
be discerned from fixed samples alone. However, imaging cell-and tissue-level processes in …

The Hippo pathway is an evolutionarily conserved regulator of tissue growth. Multiple Hippo
signaling components are regulated via proteolytic degradation. However, how these …

Microfluidic devices provide a platform for analyzing both natural and synthetic multicellular
systems. Currently, substantial capital investment and expertise are required for creating …

Regulation of microtubule stability is crucial for the maintenance of cell structure and
function. While the acetylation of α-tubulin lysine 40 by acetylase has been implicated in the …

Sensory neuron numbers and positions are precisely organized to accurately map
environmental signals in the brain. This precision emerges from biochemical processes …

In this chapter, I present a method for the ex vivo cultivation and live imaging of Drosophila
imaginal disc explants using low concentrations of the steroid hormone 20 …

We consider the non-linear elastic behavior of the wing disc of the Drosophila larva. In
stretching experiments, this epithelial tissue shows a highly non-linear force–displacement …

Several techniques have been developed to study specific gene function in loss-of-function
situations. In Drosophila melanoga st er, RNAi and the generation of mutant clones are …