Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and
spheroids are different 3D cell culture models that can be cultured with different techniques …

Organoids are microscopic self-organizing, three-dimensional structures that are grown from
stem cells in vitro. They recapitulate many structural and functional aspects of their in vivo …

Ovarian cancer (OC) is a heterogeneous disease usually diagnosed at a late stage.
Experimental in vitro models that faithfully capture the hallmarks and tumor heterogeneity of …

Colorectal cancer (CRC) patient-derived organoids predict responses to chemotherapy.
Here we used them to investigate relapse after treatment. Patient-derived organoids expand …

The intestinal epithelium withstands continuous mechanical, chemical and biological insults
despite its single-layered, simple epithelial structure. The crypt–villus tissue architecture in …

The recent advances in in vitro 3D culture technologies, such as organoids, have opened
new avenues for the development of novel, more physiological human cancer models. Such …

Organoids are in vitro–cultured three-dimensional structures that recapitulate key aspects of
in vivo organs. They can be established from pluripotent stem cells and from adult stem …

Most patients with colorectal cancer die as a result of the disease spreading to other organs.
However, no prevalent mutations have been associated with metastatic colorectal cancers …

The cancer stem cell (CSC) concept was proposed four decades ago, and states that tumor
growth, analogous to the renewal of healthy tissues, is fueled by small numbers of dedicated …

The CD155/TIGIT axis can be co-opted during immune evasion in chronic viral infections
and cancer. Pancreatic adenocarcinoma (PDAC) is a highly lethal malignancy, and immune …