Mechanisms of germ cell specification across the metazoans: epigenesis and preformation

CG Extavour, M Akam - 2003 - journals.biologists.com
Germ cells play a unique role in gamete production, heredity and evolution. Therefore, to
understand the mechanisms that specify germ cells is a central challenge in developmental …

RNA granules in germ cells

E Voronina, G Seydoux… - Cold Spring …, 2011 - cshperspectives.cshlp.org
“Germ granules” are cytoplasmic, nonmembrane-bound organelles unique to germline.
Germ granules share components with the P bodies and stress granules of somatic cells, but …

Comprehensive single-cell transcriptional profiling of a multicellular organism

J Cao, JS Packer, V Ramani, DA Cusanovich, C Huynh… - Science, 2017 - science.org
To resolve cellular heterogeneity, we developed a combinatorial indexing strategy to profile
the transcriptomes of single cells or nuclei, termed sci-RNA-seq (single-cell combinatorial …

[HTML][HTML] A user's guide for phase separation assays with purified proteins

S Alberti, S Saha, JB Woodruff, TM Franzmann… - Journal of molecular …, 2018 - Elsevier
The formation of membrane-less organelles and compartments by protein phase separation
is an important way in which cells organize their cytoplasm and nucleoplasm. In vitro phase …

Germline P granules are liquid droplets that localize by controlled dissolution/condensation

CP Brangwynne, CR Eckmann, DS Courson… - Science, 2009 - science.org
In sexually reproducing organisms, embryos specify germ cells, which ultimately generate
sperm and eggs. In Caenorhabditis elegans, the first germ cell is established when RNA and …

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

JT Wang, J Smith, BC Chen, H Schmidt, D Rasoloson… - Elife, 2014 - elifesciences.org
RNA granules have been likened to liquid droplets whose dynamics depend on the
controlled dissolution and condensation of internal components. The molecules and …

[HTML][HTML] A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish

S Houwing, LM Kamminga, E Berezikov… - Cell, 2007 - cell.com
Piwi proteins specify an animal-specific subclass of the Argonaute family that, in vertebrates,
is specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is …

The human VASA gene is specifically expressed in the germ cell lineage

DH Castrillon, BJ Quade, TY Wang… - Proceedings of the …, 2000 - National Acad Sciences
To understand the origins and function of the human germ cell lineage and to identify germ
cell-specific markers we have isolated a human ortholog of the Drosophila gene vasa. The …

The mouse homolog of Drosophila Vasa is required for the development of male germ cells

SS Tanaka, Y Toyooka, R Akasu… - Genes & …, 2000 - genesdev.cshlp.org
Restricted expression of a mouse Vasa homolog gene (Mvh) expression is first detected in
primordial germ cells (PGCs) after colonization of the genital ridges. Subsequently, Mvh is …

Not your father's planarian: a classic model enters the era of functional genomics

PA Newmark, AS Alvarado - Nature Reviews Genetics, 2002 - nature.com
Freshwater planarians were a classic model for studying the problems of development and
regeneration. However, as attention shifted towards animals with more rigid developmental …