T cell help induces Myc transcriptional bursts in germinal center B cells during positive selection
S Kagan Ben Tikva, N Gurwitz, E Sivan, D Hirsch… - Science …, 2024 - science.org
Antibody affinity maturation occurs in secondary lymphoid organs within germinal centers
(GCs). At these sites, B cells mutate their antibody-encoding genes in the dark zone …
(GCs). At these sites, B cells mutate their antibody-encoding genes in the dark zone …
[HTML][HTML] Protocol for single-molecule fluorescence in situ hybridization for intact pancreatic tissue
L Farack, S Itzkovitz - STAR protocols, 2020 - Elsevier
We describe an optimized smFISH protocol for the intact pancreas. The protocol is adapted
from Lyubimova et al.(2013), a generic tissue smFISH protocol that works for most tissues …
from Lyubimova et al.(2013), a generic tissue smFISH protocol that works for most tissues …
Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single-Molecule FISH and Immunofluorescence
Single-molecule fluorescent in situ hybridization (smFISH) enables the detection and
quantification of endogenous mRNAs within intact fixed cells. This method utilizes tens of …
quantification of endogenous mRNAs within intact fixed cells. This method utilizes tens of …
[HTML][HTML] Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing
Objectives Until recently, communication between neighboring cells in islets of Langerhans
was overlooked by genomic technologies, which require rigorous tissue dissociation into …
was overlooked by genomic technologies, which require rigorous tissue dissociation into …