Antibiotic resistance: turning evolutionary principles into clinical reality

DI Andersson, NQ Balaban, F Baquero… - FEMS microbiology …, 2020 - academic.oup.com
Antibiotic resistance is one of the major challenges facing modern medicine worldwide. The
past few decades have witnessed rapid progress in our understanding of the multiple factors …

Roadmap to the bioanalytical testing of COVID-19: from sample collection to disease surveillance

A Hosseini, R Pandey, E Osman, A Victorious, F Li… - ACS …, 2020 - ACS Publications
The disease caused by SARS-CoV-2, coronavirus disease 2019 (COVID-19), has led to a
global pandemic with tremendous mortality, morbidity, and economic loss. The current lack …

A novel “signal on-off-super on” sandwich-type aptamer sensor of CRISPR-Cas12a coupled voltage enrichment assay for VEGF detection

G Yuan, X Xia, J Zhang, J Huang, F Xie, X Li… - Biosensors and …, 2023 - Elsevier
Vascular endothelial growth factor (VEGF) plays an important role in atherosclerosis, and
the detection of VEGF is critical for the prevention, monitoring, and diagnosis of …

Multivalent duplexed-aptamer networks regulated a CRISPR-Cas12a system for circulating tumor cell detection

Z Lv, Q Wang, M Yang - Analytical Chemistry, 2021 - ACS Publications
Although circulating tumor cells (CTCs) have great potential to act as the mini-invasive liquid
biopsy cancer biomarker, a rapid and sensitive CTC detection method remains lacking …

Highly sensitive and rapid detection of SARS‐CoV‐2 via a portable CRISPR‐Cas13a‐based lateral flow assay

H Liu, S Chang, S Chen, Y Du, H Wang… - Journal of Medical …, 2022 - Wiley Online Library
To rapidly identify individuals infected with severe acute respiratory syndrome coronavirus‐2
(SARS‐CoV‐2) and control the spread of coronavirus disease (COVID‐19), there is an …

A rapid and high-throughput Helicobacter pylori RPA-CRISPR/Cas12a-based nucleic acid detection system

H Liu, J Wang, X Hu, X Tang, C Zhang - Clinica Chimica Acta, 2023 - Elsevier
Background Helicobacter pylori lives in the human stomach and causes gastric cancer and
other gastric diseases. The development of molecular technology has facilitated low-cost …

CRISPR-cas technology: A key approach for SARS-CoV-2 detection

L Fang, L Yang, M Han, H Xu, W Ding… - … in bioengineering and …, 2023 - frontiersin.org
The CRISPR (Clustered Regularly Spaced Short Palindromic Repeats) system was first
discovered in prokaryotes as a unique immune mechanism to clear foreign nucleic acids. It …

Point-of-care testing for infectious diseases based on class 2 CRISPR/Cas technology

SJ Chen, CI Rai, SC Wang, YC Chen - Diagnostics, 2023 - mdpi.com
The early detection of infectious diseases and microorganisms is critical for effective disease
treatment, control, and prevention. Currently, nucleic acid testing and antigen–antibody …

CRISPR/Cas9-based lateral flow and fluorescence diagnostics

MJ Osborn, A Bhardwaj, SP Bingea, F Knipping… - Bioengineering, 2021 - mdpi.com
Clustered regularly interspaced short palindromic repeat (CRISPR/Cas) proteins can be
designed to bind specified DNA and RNA sequences and hold great promise for the …

Effective use of linear DNA in cell-free expression systems

MA McSweeney, MP Styczynski - Frontiers in Bioengineering and …, 2021 - frontiersin.org
Cell-free expression systems (CFEs) are cutting-edge research tools used in the
investigation of biological phenomena and the engineering of novel biotechnologies. While …