Lipoarabinomannan as a point-of-care assay for diagnosis of tuberculosis: how far are we to use it?

J Flores, JC Cancino, L Chavez-Galan - Frontiers in microbiology, 2021 - frontiersin.org
Tuberculosis (TB) is still a severe public health problem; the current diagnostic tests have
limitations that delay treatment onset. Lipoarabinomannan (LAM) is a glycolipid that is a …

Cargoes of exosomes function as potential biomarkers for Mycobacterium tuberculosis infection

N Wang, Y Yao, Y Qian, D Qiu, H Cao, H Xiang… - Frontiers in …, 2023 - frontiersin.org
Exosomes as double-membrane vesicles contain various contents of lipids, proteins,
mRNAs and non-coding RNAs, and involve in multiple physiological processes, for instance …

Sensitive urine immunoassay for visualization of lipoarabinomannan for noninvasive tuberculosis diagnosis

P Chen, Y Meng, T Liu, W Peng, Y Gao, Y He, R Qu… - ACS …, 2023 - ACS Publications
Lipoarabinomannan (LAM) is a prospective noninvasive biomarker for tuberculosis (TB)
diagnosis. Here, we report a visual immunoassay of high sensitivity for detecting LAM in …

Diagnosis of genitourinary tuberculosis: detection of mycobacterial lipoarabinomannan and MPT-64 biomarkers within urine extracellular vesicles by nano-based …

E Kamra, T Prasad, A Rais, B Dahiya, A Sheoran… - Scientific Reports, 2023 - nature.com
We detected a cocktail of Mycobacterium tuberculosis lipoarabinomannan (LAM) and MPT-
64 biomarkers within urine extracellular vesicles (EVs) of genitourinary TB (GUTB) patients …

Diagnosis of tuberculosis by nanoparticle-based immuno-PCR assay based on mycobacterial MPT64 and CFP-10 detection

B Dahiya, T Prasad, V Singh, A Khan, E Kamra… - …, 2020 - Taylor & Francis
Aim: To improve the diagnostic accuracy of immuno-PCR (I-PCR) in tuberculosis (TB)
patients by using functionalized gold nanoparticles (AuNPs) coupled with detection …

Emerging role of exosomes in tuberculosis: from immunity regulations to vaccine and immunotherapy

YF Sun, J Pi, JF Xu - Frontiers in Immunology, 2021 - frontiersin.org
Exosomes are cell-derived nanovesicles carrying protein, lipid, and nucleic acid for
secreting cells, and act as significant signal transport vectors for cell-cell communication and …

Extracellular vesicles in mycobacteria and tuberculosis

C Mehaffy, JM Ryan, NA Kruh-Garcia… - Frontiers in Cellular and …, 2022 - frontiersin.org
Tuberculosis (TB) remains a public health issue causing millions of infections every year. Of
these, about 15% ultimately result in death. Efforts to control TB include development of new …

Insight into diagnosis of female genital tuberculosis

B Dahiya, E Kamra, D Alam, M Chauhan… - Expert Review of …, 2022 - Taylor & Francis
Introduction Female genital tuberculosis (TB) is a common form of extrapulmonary TB
(EPTB) with varied clinical presentations, ie. infertility, pelvic pain, and menstrual …

Detection of potential biomarkers associated with outrageous diseases and environmental pollutants by nanoparticle-based immuno-PCR assays

B Dahiya, PK Mehta - Analytical biochemistry, 2019 - Elsevier
Immuno-polymerase chain reaction (I-PCR) assay with advantages of both enzyme-linked
immunosorbent assay (ELISA) and PCR exhibits several-fold enhanced sensitivity in …

Diagnosis of extrapulmonary tuberculosis by GeneXpert MTB/RIF Ultra assay

B Dahiya, N Mehta, A Soni… - Expert Review of Molecular …, 2023 - Taylor & Francis
Introduction Diagnosis of extrapulmonary tuberculosis (EPTB) is an arduous task owing to
different anatomical locations, unusual clinical presentations, and sparse bacillary load in …