Obtaining high quantities of a specific protein directly from native sources is often
challenging, particularly when dealing with human proteins. To overcome this obstacle …

Background Labeling efficiency is a crucial parameter in fluorescence applications,
especially when studying biomolecular interactions. Current approaches for estimating the …

Pichia pastoris is a methylotrophic yeast capable of metabolizing methanol as its sole
carbon source. Growth in methanol-containing medium results in dramatic induction of …

Abstract Pertactin (PRN), a non-fimbrial outer membrane protein of Bordetella pertussis, is
the limiting component of the acellular pertussis vaccine because of its low concentration …

Recovery of intracellular proteins requires disruption of the host cell before the target protein
is extracted and isolated. Disruption methods vary depending on the type of cells, the total …

Background Labeling efficiency is a crucial parameter in fluorescence applications,
especially when studying biomolecular interactions. Current approaches for estimating the …

Downstream processing of trypsin synthesis, which includes purification, is a major issue
due to high complexity of the biosuspension itself and the stress sensitivity of the desired …