Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography

X Wu, N Zhou, Y Chen, J Sun, L Lu, Q Chen… - Light: Science & …, 2024 - nature.com
Lens-free on-chip microscopy is a powerful and promising high-throughput computational
microscopy technique due to its unique advantage of creating high-resolution images across …

Deep learning massively accelerates super-resolution localization microscopy

W Ouyang, A Aristov, M Lelek, X Hao, C Zimmer - Nature biotechnology, 2018 - nature.com
The speed of super-resolution microscopy methods based on single-molecule localization,
for example, PALM and STORM, is limited by the need to record many thousands of frames …

Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging

S Fu, W Shi, T Luo, Y He, L Zhou, J Yang, Z Yang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …

Technological advances in super-resolution microscopy to study cellular processes

C Bond, AN Santiago-Ruiz, Q Tang, M Lakadamyali - Molecular Cell, 2022 - cell.com
Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

CaMKII activation persistently segregates postsynaptic proteins via liquid phase separation

T Hosokawa, PW Liu, Q Cai, JS Ferreira, F Levet… - Nature …, 2021 - nature.com
Transient information input to the brain leads to persistent changes in synaptic circuits,
contributing to the formation of memory engrams. Pre-and postsynaptic structures undergo …

Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging

W Zhao, S Zhao, Z Han, X Ding, G Hu, L Qu… - Nature …, 2023 - nature.com
High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision
profiling in a wide range of biomedical applications. However, current SR methods require …

A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei

JM Phillip, KS Han, WC Chen, D Wirtz, PH Wu - Nature protocols, 2021 - nature.com
Cell morphology encodes essential information on many underlying biological processes. It
is commonly used by clinicians and researchers in the study, diagnosis, prognosis, and …

MemBright: a family of fluorescent membrane probes for advanced cellular imaging and neuroscience

M Collot, P Ashokkumar, H Anton, E Boutant… - Cell chemical …, 2019 - cell.com
The proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the
cell. Herein, we developed MemBright, a family of six cyanine-based fluorescent turn-on PM …