The cyclin-dependent protein kinase (CDK) encoded by CDC28 is the master regulator of
cell division in the budding yeast Saccharomyces cerevisiae. By mechanisms that, for the …

Eukaryotic DNA replication is regulated to ensure all chromosomes replicate once and only
once per cell cycle. Replication begins at many origins scattered along each chromosome …

Evidence obtained from studies with yeast and Xenopusindicate that the initiation of DNA
replication is a multistep process. The origin recognition complex (ORC), Cdc6p, and …

In eukaryotes a cell-cycle control termed a checkpoint causes arrest in the S or G2 phases
when chromosomes are incompletely replicated or damaged. Previously, we showed in …

In fission yeast, the M‐phase inducing kinase, a complex of p34cdc2 and cyclin B, is
maintained in an inhibited state during interphase due to the phosphorylation of Cdc2 at …

In budding yeast genes that encode G1 cyclins and proteins involved in DNA synthesis are
transcriptionally activated in late G1. A transcription factor, called SBF, is composed of Swi4 …

B-type cyclin destruction is necessary for exit from mitosis and the initiation of a new cell
cycle. Through the isolation of mutants, we have identified three essential yeast genes …

The four-subunit DNA polymerase alpha-primase complex is unique in its ability to
synthesize DNA chains de novo, and some in vitro data suggest its involvement in initiation …

ORIGINS of DNA replication in Saccharomyces cerevisiae are bound by two protein
complexes during the cell cycle1, 2. Post-replicative complexes closely resemble those …

The initiation of DNA replication in eukaryotic cells is a highly regulated process that leads to
the duplication of the genetic information for the next cell generation. This requires the …