Strains of bacteria resistant to antibiotics, particularly those that are multiresistant, are an
increasing major health care problem around the world. It is now abundantly clear that both …

Since the first two complete bacterial genome sequences were published in 1995, the
science of bacteria has dramatically changed. Using third-generation DNA sequencing, it is …

Long-read-only bacterial genome assemblies usually contain residual errors, most
commonly homopolymer-length errors. Short-read polishing tools can use short reads to fix …

Antibiotic resistance genes (ARGs) have accelerated microbial threats to human health in
the last decade. Many genes can confer resistance, but evaluating the relative health risks of …

Carbapenem-resistant Acinetobacter baumannii (CRAB) has emerged as a major global
pathogen with limited treatment options. No new antibiotic chemical class with activity …

Transposition has a key role in reshaping genomes of all living organisms. Insertion
sequences of IS200/IS605 and IS607 families are among the simplest mobile genetic …

Objectives Antimicrobial resistance (AMR) in clinically relevant bacteria is a growing threat
to public health globally. In these bacteria, antimicrobial resistance genes are often …

It is well established that plasmids play an important role in the dissemination of
antimicrobial resistance (AMR) genes; however, little is known about the role of the …

Bacterial mobile genetic elements (MGEs) encode functional modules that perform both core
and accessory functions for the element, the latter of which are often only transiently …

The Illumina DNA sequencing platform generates accurate but short reads, which can be
used to produce accurate but fragmented genome assemblies. Pacific Biosciences and …