Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

Cell manipulation in droplets has emerged as one of the great successes of microfluidic
technologies, with the development of single-cell screening. However, the droplet format has …

Despite tremendous advancements in technologies and resources, drug discovery still
remains a tedious and expensive process. Though most cells are cultured using 2D …

Droplet microfluidic techniques have long been known as a high-throughput approach for
cell manipulation. The capacity to compartmentalize cells into picolitre droplets in …

Abstract 3D in vitro model systems such as spheroids and organoids provide an opportunity
to extend the physiological understanding using recapitulated tissues that mimic …

Cancer still ranks as a leading cause of mortality worldwide. Although considerable efforts
have been dedicated to anticancer therapeutics, progress is still slow, partially due to the …

Two-dimensional (2D) cell culture techniques are commonly employed to investigate
biophysical and biochemical cellular responses. However, these culture methods, having …

Cell spheroids (esp. organoids) as 3D culture platforms are popular models for representing
cell–cell and cell–extracellular matrix (ECM) interactions, bridging the gap between 2D cell …

Simple Summary Induced pluripotent stem cells (iPSCs) that can be genetically engineered
and differentiated into different types of immune cells, providing an unlimited resource for …

Brain organoids are self-assembled three-dimensional aggregates with brain-like cell types
and structures and have emerged as new model systems that can be used to investigate …