Lentiviral vectors (LVs) are potent tools for the delivery of genes of interest into mammalian
cells and are now commonly utilised within the growing field of cell and gene therapy for the …

Since techniques for cardiomyocyte isolation were first developed 35years ago, experiments
on single myocytes have yielded great insight into their cellular and sub-cellular physiology …

Emerging and reemerging infectious diseases have a strong negative impact on public
health. However, because many of these pathogens must be handled in biosafety level, 3 or …

Abstract α-Galactosylceramide (αGalCer) stimulates NKT cells and has antitumor activity in
mice. Murine NKT cells may directly kill tumor cells and induce NK cell cytotoxicity, but the …

The goal of the present study was to develop an efficient transient transfection method for
large-scale production of high titer lentivirus vector stocks of eight different pseudotypes. The …

Background Lentiviral vectors have emerged as efficient vehicles for transgene delivery in
both dividing and non-dividing cells. A number of different modifications in vector design …

Dystrophic epidermolysis bullosa (DEB) is a family of inherited mechano-bullous disorders
caused by mutations in the human type VII collagen gene (COL7A1). Individuals with DEB …

Background Lentiviral vectors (LV) offer several advantages over other gene delivery
vectors. Their potential for the integration and long‐term expression of therapeutic genes …

Although much progress has been made in identifying the mechanisms that trigger
endothelial activation and inflammatory cell recruitment during atherosclerosis, less is …

Generation of high titer lentiviral stocks and efficient virus concentration are central to
maximize the utility of lentiviral technology. Here we evaluate published protocols for …