CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …

RNA-guided systems, which use complementarity between a guide RNA and target nucleic
acid sequences for recognition of genetic elements, have a central role in biological …

As a miniature RNA-guided endonuclease, IscB is presumed to be the ancestor of Cas9 and
to share similar functions. IscB is less than half the size of Cas9 and thus more suitable for in …

Plant metabolism and development are a reflection of the orderly expression of genetic
information intertwined with the environment interactions. Genome editing is the cornerstone …

The miniature CRISPR-Cas12f nucleases enable efficient delivery via cargo-size-limited
vehicles, thereby showing promise for in vivo therapeutic applications. Acidibacillus …

Abstract OMEGA RNA (ωRNA)-guided endonuclease IscB, the evolutionary ancestor of
Cas9, is an attractive system for in vivo genome editing because of its compact size and …

Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has
been widely used for genome editing in mammalian cells and plants due to its distinct …

Summary The CRISPR/Cas type V‐I is a family of programmable nuclease systems that
prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this …

The IscB proteins, as the ancestors of Cas9 endonuclease, hold great promise due to their
small size and potential for diverse genome editing. However, their activity in mammalian …

The discovery of CRISPR-Cas systems has paved the way for advanced gene editing tools.
However, traditional Cas discovery methods relying on sequence similarity may miss distant …