Methods for copy number aberration detection from single-cell DNA-sequencing data

XF Mallory, M Edrisi, N Navin, L Nakhleh - Genome biology, 2020 - Springer
Copy number aberrations (CNAs), which are pathogenic copy number variations (CNVs),
play an important role in the initiation and progression of cancer. Single-cell DNA …

Whole-genome CNV analysis: advances in computational approaches

M Pirooznia, FS Goes, PP Zandi - Frontiers in genetics, 2015 - frontiersin.org
Accumulating evidence indicates that DNA copy number variation (CNV) is likely to make a
significant contribution to human diversity and also play an important role in disease …

CNVkit: genome-wide copy number detection and visualization from targeted DNA sequencing

E Talevich, AH Shain, T Botton… - PLoS computational …, 2016 - journals.plos.org
Germline copy number variants (CNVs) and somatic copy number alterations (SCNAs) are
of significant importance in syndromic conditions and cancer. Massively parallel sequencing …

Clonal expansion and diversification of cancer-associated mutations in endometriosis and normal endometrium

K Suda, H Nakaoka, K Yoshihara, T Ishiguro, R Tamura… - Cell reports, 2018 - cell.com
Endometriosis is characterized by ectopic endometrial-like epithelium and stroma, of which
molecular characteristics remain to be fully elucidated. We sequenced 107 ovarian …

Whole-organism clone tracing using single-cell sequencing

A Alemany, M Florescu, CS Baron, J Peterson-Maduro… - Nature, 2018 - nature.com
Embryonic development is a crucial period in the life of a multicellular organism, during
which limited sets of embryonic progenitors produce all cells in the adult body. Determining …

Heterogeneity of neuroblastoma cell identity defined by transcriptional circuitries

V Boeva, C Louis-Brennetot, A Peltier, S Durand… - Nature …, 2017 - nature.com
Neuroblastoma is a tumor of the peripheral sympathetic nervous system, derived from
multipotent neural crest cells (NCCs). To define core regulatory circuitries (CRCs) controlling …

Mapping and phasing of structural variation in patient genomes using nanopore sequencing

M Cretu Stancu, MJ Van Roosmalen, I Renkens… - Nature …, 2017 - nature.com
Despite improvements in genomics technology, the detection of structural variants (SVs)
from short-read sequencing still poses challenges, particularly for complex variation. Here …

Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)

C Chen, D Xing, L Tan, H Li, G Zhou, L Huang, XS Xie - Science, 2017 - science.org
Single-cell genomics is important for biology and medicine. However, current whole-
genome amplification (WGA) methods are limited by low accuracy of copy-number variation …

Tumorigenicity and genetic profiling of circulating tumor cells in small-cell lung cancer

CL Hodgkinson, CJ Morrow, Y Li, RL Metcalf… - Nature medicine, 2014 - nature.com
Small-cell lung cancer (SCLC), an aggressive neuroendocrine tumor with early
dissemination and dismal prognosis, accounts for 15–20% of lung cancer cases and∼ …

DeLTA: Automated cell segmentation, tracking, and lineage reconstruction using deep learning

JB Lugagne, H Lin, MJ Dunlop - PLoS computational biology, 2020 - journals.plos.org
Microscopy image analysis is a major bottleneck in quantification of single-cell microscopy
data, typically requiring human oversight and curation, which limit both accuracy and …