[HTML][HTML] Islet product characteristics and factors related to successful human islet transplantation from the Collaborative Islet Transplant Registry (CITR) 1999–2010
AN Balamurugan, B Naziruddin, A Lockridge… - American Journal of …, 2014 - Elsevier
The Collaborative Islet Transplant Registry (CITR) collects data on clinical islet isolations
and transplants. This retrospective report analyzed 1017 islet isolation procedures …
and transplants. This retrospective report analyzed 1017 islet isolation procedures …
Identifying effective enzyme activity targets for recombinant class I and class II collagenase for successful human islet isolation
AN Balamurugan, ML Green, AG Breite… - Transplantation …, 2016 - journals.lww.com
Background Isolation following a good manufacturing practice-compliant, human islet
product requires development of a robust islet isolation procedure where effective limits of …
product requires development of a robust islet isolation procedure where effective limits of …
[HTML][HTML] Beneficial effect of recombinant rC1rC2 collagenases on human islet function: Efficacy of low-dose enzymes on pancreas digestion and yield
G Loganathan, V Subhashree, AG Breite… - American Journal of …, 2018 - Elsevier
A high number of human islets can be isolated by using modern purified tissue dissociation
enzymes; however, this requires the use of> 20 Wunsch units (WU)/g of pancreas for …
enzymes; however, this requires the use of> 20 Wunsch units (WU)/g of pancreas for …
Layer-by-layer immobilized catalase on electrospun nanofibrous mats protects against oxidative stress induced by hydrogen peroxide
R Huang, H Deng, T Cai, Y Zhan… - Journal of …, 2014 - ingentaconnect.com
Catalase, a kind of redox enzyme and generally recognized as an efficient agent for
protecting cells against hydrogen peroxide (H2O2)-induced cytotoxicity. The immobilization …
protecting cells against hydrogen peroxide (H2O2)-induced cytotoxicity. The immobilization …
Clostripain, the missing link in the enzyme blend for efficient human islet isolation
M Ståhle, A Foss, B Gustafsson, M Lempinen… - Transplantation …, 2015 - journals.lww.com
Background Effective digestive enzymes are crucial for successful islet isolation.
Supplemental proteases are essential as they synergize with collagenase for effective …
Supplemental proteases are essential as they synergize with collagenase for effective …
Multicenter assessment of animal-free collagenase AF-1 for human islet isolation
D Brandhorst, G Parnaud, A Friberg… - Cell …, 2017 - journals.sagepub.com
Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have
recently become available for human islet isolation. This report describes the initial …
recently become available for human islet isolation. This report describes the initial …
Optimizing porcine islet isolation to markedly reduce enzyme consumption without sacrificing islet yield or function
RW Holdcraft, ML Green, AG Breite… - Transplantation …, 2016 - journals.lww.com
Background Human allogeneic islet transplantation for treatment of type 1 diabetes provides
numerous clinical benefits, such as fewer episodes of hypoglycemic unawareness and …
numerous clinical benefits, such as fewer episodes of hypoglycemic unawareness and …
Collagenases in pancreatic islet isolation
I Fathi, M Goto - … , Bioengineering, and Regeneration of the Endocrine …, 2020 - Elsevier
Collagenases, essential elements in experimental and clinical islet isolation, have been the
subject of numerous investigations over the last three decades in order to standardize and …
subject of numerous investigations over the last three decades in order to standardize and …