Proteins are the main targets of most drugs; however, system-wide methods to monitor
protein activity and function are still underused in drug discovery. Novel biochemical …

Mass spectrometry (MS)-based proteomics has become a powerful technology to quantify
the entire complement of proteins in cells or tissues. Here, we review challenges and recent …

Liquid chromatography (LC) coupled with data-independent acquisition (DIA) mass
spectrometry (MS) has been increasingly used in quantitative proteomics studies. Here, we …

Implementing precision medicine hinges on the integration of omics data, such as
proteomics, into the clinical decision-making process, but the quantity and diversity of …

Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging
and deep sequencing. However, proteins are the main drivers of cellular function and in …

Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-
state heterogeneity and distinct cellular signaling patterns that remain obscured with …

Major aims of single-cell proteomics include increasing the consistency, sensitivity and
depth of protein quantification, especially for proteins and modifications of biological interest …

We present an easy-to-use integrated software suite, DIA-NN, that exploits deep neural
networks and new quantification and signal correction strategies for the processing of data …

Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific
functional networks underlying biological processes. Here, we present a streamlined …

Declining tissue nicotinamide adenine dinucleotide (NAD) levels are linked to ageing and its
associated diseases. However, the mechanism for this decline is unclear. Here, we show …